Construction of vascular graft with circumferentially oriented microchannels for improving artery regeneration
Construction of vascular graft with circumferentially oriented microchannels for improving artery regeneration
复制标题
构建具有周向微通道的血管移植物以改善动脉再生
DOI：
10.1016/j.biomaterials.2020.119922
复制
发表时间：
2020-06-01
期刊：
影响因子：
14
通讯作者：
Zhu, Meifeng
中科院分区：
文献类型：
--
作者：
Wu, Pingli;Wang, Lina;Zhu, Meifeng
设计和制造具有三维（3D）拓扑线索的支架，其诱导与天然组织相当的新组织再生仍然是科学和临床领域的主要挑战。在这里，我们开发了一个精心设计的血管移植物与三维高度互连和周向取向的微通道，通过使用牺牲糖微纤维浸出方法。微通道结构能够促进血管平滑肌细胞（VSMCs）的迁移、定向排列、伸长和收缩表型表达。植入大鼠主动脉缺损模型后，血管移植物中的微通道同时促进了VSMCs的浸润和排列，细胞外基质（ECM）的定向沉积，以及巨噬细胞的募集和极化。这些积极的结果也为EC的生长提供了保护和支持，并最终加速了内皮化。本研究为制备具有诱导动脉再生能力的支架提供了一种新的策略，并可进一步推广应用于其他定向支架的制备，以引导组织定向原位再生。
Design and fabrication of scaffolds with three-dimensional (3D) topological cues inducing regeneration of the neo-tissue comparable to native one remains a major challenge in both scientific and clinical fields. Here, we developed a well-designed vascular graft with 3D highly interconnected and circumferentially oriented microchannels by using the sacrificial sugar microfiber leaching method. The microchannels structure was capable of promoting the migration, oriented arrangement, elongation, and the contractile phenotype expression of vascular smooth muscle cells (VSMCs) in vitro. After implantation into the rat aorta defect model, the microchannels in vascular grafts simultaneously improved the infiltration and aligned arrangement of VSMCs and the oriented deposition of extracellular matrix (ECM), as well as the recruitment and polarization of macrophages. These positive results also provided protection and support for ECs growth, and ultimately accelerated the endothelialization. Our research provides a new strategy for the fabrication of grafts with the capability of inducing arterial regeneration, which could be further extended to apply in preparing other kinds of oriented scaffolds aiming to guide oriented tissue in situ regeneration.