Involvement of spectrin in the maintenance of phase-state asymmetry in the erythrocyte membrane
Involvement of spectrin in the maintenance of phase-state asymmetry in the erythrocyte membrane
复制标题
血影蛋白参与维持红细胞膜相态不对称性
DOI：
10.1016/0092-8674(82)90277-x
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发表时间：
1982
期刊：
影响因子：
64.5
通讯作者：
R. Schlegel
中科院分区：
文献类型：
--
作者：
P. Williamson;J. Bateman;K. Kozarsky;K. Mattocks;Neal Hermanowicz;Hye‐Ryun Choe;R. Schlegel
荧光探针mercury 540不染色正常人或鼠红细胞的质膜，也不染色遗传异常的人球形红细胞的质膜。然而，它确实在一些系统中染色红细胞膜，其中底层血影蛋白网络被改变或缺失。由于mercury 540对流体相脂质双层的亲和力更大，这些结果表明，红细胞膜的外部小叶在内部血影蛋白网络改变或丧失后变得更加无序。磷脂酶A2消化的膜磷脂的transbilayer安排的分析表明，红细胞膜的脂质成分的不对称性是负责两个脂质小叶之间的相态不对称性，并需要血影蛋白来维持这种不对称性和凝胶样状态的外部小叶。
The fluorescent probe merocyanine 540 does not stain the plasma membrane of normal human or murine erythrocytes, nor of genetically abnormal human spherocytic erythrocytes. It does, however, stain erythrocyte membranes in several systems in which the underlying spectrin network is altered or missing. Because of the greater affinity of merocyanine 540 for fluid--phase lipid bilayers, these results suggest that the external leaflet of erythrocyte membranes becomes more disordered upon alteration or loss of the internal spectrin network. Analysis of the transbilayer arrangement of membrane phospholipids by digestion with phospholipase A2 suggests that lipid compositional asymmetry of the erythrocyte membrane is responsible for a phasestate asymmetry between the two lipid leaflets, and that spectrin is required to maintain this asymmetry and the gel-like state of the external leaflet.
部分遗传性球形红细胞增多症家系红细胞膜骨架分子缺陷的鉴定。
影响因子：
20.3
作者：
Goodman,SR;Shiffer,KA;Casoria,LA;Eyster,ME
通讯作者：
Eyster,ME