Isolation and characterization of functional mammary gland stem cells
Isolation and characterization of functional mammary gland stem cells
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DOI：
10.1046/j.1365-2184.36.s.1.3.x
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发表时间：
2003-10-01
影响因子：
8.5
通讯作者：
Rosen, JM
中科院分区：
文献类型：
--
作者：
Welm, B;Behbod, F;Rosen, JM
在过去几年中，包括乳腺在内的几种组织的干细胞生物学取得了重大进展。最近的进展干细胞的命运决定，分子标记物，信号通路和生态位在造血，神经元和肌肉组织的相互作用，可能会提供平行洞察乳腺上皮干细胞的生物学。利用类似的方法来分离和表征造血干细胞和表皮干细胞，我们已经确定了一个乳腺上皮细胞群与几个干/祖细胞的质量。本文就乳腺上皮干/祖细胞在基因工程小鼠模型中的研究进展作一综述。我们还讨论了几个潜在的分子标记，包括干细胞抗原-1（Sca-1），这可能是有用的功能性乳腺上皮干/祖细胞的分离和基因工程小鼠模型中的肿瘤病因学和表型的分析。在不同的转基因乳腺肿瘤模型中，Sca-1表达水平以及包括角蛋白-6在内的几种其他假定的祖细胞标志物具有显著改变的表达谱。这些数据表明，乳腺癌小鼠模型的异质性可能部分反映了不同祖细胞的选择或扩增。
Significant advances in the stem-cell biology of several tissues, including the mammary gland, have occurred over the past several years. Recent progress on stem-cell fate determination, molecular markers, signalling pathways and niche interactions in haematopoietic, neuronal and muscle tissue may provide parallel insight into the biology of mammary epithelial stem cells. Taking advantage of approaches similar to those employed to isolate and characterize haematopoietic and epidermal stem cells, we have identified a mammary epithelial cell population with several stem/progenitor cell qualities. In this article, we review some recent data on mammary epithelial stem/progenitor cells in genetically engineered mouse models. We also discuss several potential molecular markers, including stem-cell antigen-1 (Sca-1), which may be useful for both the isolation of functional mammary epithelial stem/progenitor cells and the analysis of tumour aetiology and phenotype in genetically engineered mouse models. In different transgenic mammary tumour models, Sca-1 expression levels, as well as several other putative markers of progenitors including keratin-6, possess dramatically altered expression profiles. These data suggest that the heterogeneity of mouse models of breast cancer may partially reflect the selection or expansion of different progenitors.