Cloning and sequence analysis of the eburicol 14 alpha-demethylase gene of the obligate biotrophic grape powdery mildew fungus
Cloning and sequence analysis of the eburicol 14 alpha-demethylase gene of the obligate biotrophic grape powdery mildew fungus
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DOI：
10.1016/s0378-1119(97)00141-8
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发表时间：
1997-08-11
期刊：
影响因子：
3.5
通讯作者：
CorioCostet, MF
中科院分区：
文献类型：
--
作者：
Delye, C;Laigret, F;CorioCostet, MF
为了获得关于葡萄白粉病的病原体葡萄钩丝壳（Uncinula necator）对主要杀菌剂甾醇脱甲基化抑制剂的田间抗性的分子数据，从该专性生物营养型植物病原真菌中克隆编码这些化合物的靶标（eburicol 14 α-脱甲基酶）的基因并进行测序。该单拷贝基因编码524个氨基酸的蛋白质，该蛋白质与其他已知的甾醇14 α-脱甲基酶（CYP 51）具有高度相似性。编码序列在与意大利青霉CYP 51中的内含子相同的位置处被两个短内含子中断，意大利青霉CYP 51是唯一已知的其他已鉴定出内含子的CYP 51基因。通过cDNA测序验证内含子切除。(C)1997年Elsevier Science B.V.
In order to obtain molecular data concerning field resistance of Uncinula necator, the causal agent of grape powdery mildew, to sterol demethylation inhibitors, a major group of fungicides, the gene encoding the target of these compounds (eburicol 14 alpha-demethylase) was cloned and sequenced from this obligately biotrophic phytopathogenic fungus. This single-copy gene encodes a 524 amino acid protein which displays high similarity to other known sterol 14 alpha-demethylases (CYP51s). The coding sequence is interrupted by two short introns at positions identical to introns in Penicillium italicum CYP51, which is the only other known CYP51 gene in which introns have been identified. Intron excision was verified by cDNA sequencing. (C) 1997 Elsevier Science B.V.