LANTHANUM STAINING OF THE SURFACE COAT OF CELLS
LANTHANUM STAINING OF THE SURFACE COAT OF CELLS
复制标题
细胞表面涂层的镧染色
作者：
S. M. Shea
在已经报道的用于染色细胞表面涂层的技术中，对于电子显微镜，是镧整体染色。同样，据报道，在一级戊二醛固定液中加入阳离子染料阿尔新蓝8 GX可改善许多类型细胞表面涂层的保存;然而，除非对薄切片进行复染，否则保存的涂层的电子不透明性并不高。本文表明，对于几个大鼠组织的镧染色整块是一种有效的细胞表面的电子染色，提供良好的对比度，如果顺序结合预固定在醛固定剂含有阿尔辛蓝。发现阳离子物质氯化十六烷基吡啶在增强肠上皮细胞刷状缘表面被膜材料的镧染色方面具有与阿辛蓝相似的效果。所研究的组织获得的镧染色模式与文献中报道的模式惊人地相似，文献中通过几种标准方法对组织进行染色，以在超微结构水平上证明粘液物质。这一事实和西吡氯铵对阿尔新蓝作用的再现构成了一个有说服力的经验论证，即所观察到的物质是粘多糖或粘多糖-蛋白质复合物。
Among the techniques which have been reported to stain the surface coat of cells, for electron microscopy, is lanthanum staining en bloc. Similarly, the presence of the cationic dye, Alcian blue 8GX, in a primary glutaraldehyde fixative has been reported to improve the preservation of the surface coat of cells of many types; however, the preserved coat is not very electron opaque unless thin sections are counterstained. The present paper shows that for several rat tissues lanthanum staining en bloc is an effective electron stain for the cell surface, giving excellent contrast, if combined sequentially with prefixation in an aldehyde fixative containing Alcian blue. The cationic substance cetylpyridinium chloride was found to have a similar effect to that of Alcian blue in enhancing the lanthanum staining of the surface coat material of the brush border of intestinal epithelial cells. The patterns of lanthanum staining obtained for the tissues studied strikingly resemble those reported in the literature where tissues are stained by several standard methods for demonstrating mucosubstances at the ultrastructural level. This fact and the reproduction of the effect of Alcian blue by cetylpyridinium chloride constitute a persuasive empirical argument that the material visualized is a mucopolysaccharide or mucopolysaccharide-protein complex.