Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury
Differential accumulation of mRNA for immediate early genes and heat shock genes in heart after ischaemic injury
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DOI：
10.1006/jmcc.1996.0115
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发表时间：
1996-06-01
影响因子：
5
通讯作者：
Currie, RW
中科院分区：
文献类型：
--
作者：
Plumier, JCL;Robertson, HA;Currie, RW
缺血性损伤导致热休克和即刻早期基因的表达。在此，通过在冠状动脉闭塞30分钟后缓冲液灌注的离体大鼠心脏的连续切片中进行原位杂交分析来检查这种诱导的定位。hsc 70，hsp 70，c-fos，c-jun和Erg-1的mRNA的积累被一致地定位，并且仅限于心脏的缺血区域。在缺血期结束时（无再灌注），这些基因的mRNA无法检测到。再灌注30分钟后，hsc 70，hsp 70，c-fos和c-jun的mRNA的积累是可检测的，并随着进一步再灌注而增加。在标记这些基因产物的区域内，有一个标记强度较低的中心区域，对应于坏死区。即刻早期基因产物jun-B定位于心脏的缺血和非缺血区域。这些结果表明，热休克和立即早期基因转录物积累的心脏区域，而受伤，恢复转录活性，和最小的热休克和立即早期基因转录物积累的中心区域，不恢复转录活性，是不可逆的损伤。(C)1996年学术出版社
Ischaemic injury leads to the expression of heat shock and immediate early genes. Here the localization of this induction is examined by in situ hybridization analysis in serial sections of buffer-perfused isolated rat heart after 30 min of coronary artery occlusion. The accumulation of mRNA for hsc70, hsp70, c-fos, c-jun, and Erg-1 was localized coincidently and was restricted to the ischaemic area of the heart. mRNA for these genes was undetectable at the end of the ischaemic period (no reperfusion). After 30 min of reperfusion, accumulation of mRNA for hsc70, hsp70, c-fos, and c-jun was detectable and increased with further reperfusion. Within the area labelled for these gene products was a central area of less intense labelling which corresponded to the necrotic zone. The immediate early gene product, jun-B, was localized in both the ischaemic and the non-ischaemic areas of the hearts. These results suggest that the area of the heart where heat shock and immediate early gene transcripts accumulate, while injured, recovers transcriptional activity, and that the central area where minimal heat shock and immediate early gene transcripts accumulate, does not recover transcriptional activity and is irreversibly injured. (C) 1996 Academic Press Limited