Tick feeding modulates the human skin immune landscape to facilitate tick-borne pathogen transmission.
Tick feeding modulates the human skin immune landscape to facilitate tick-borne pathogen transmission.
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DOI：
10.1172/jci161188
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发表时间：
2022-11-01
影响因子：
15.9
通讯作者：
Stary, Georg
中科院分区：
文献类型：
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作者：
Strobl, Johanna;Muendler, Verena;Mueller, Sophie;Gindl, Anna;Berent, Sara;Schoetta, Anna-Margarita;Kleissl, Lisa;Staud, Clement;Redl, Anna;Unterluggauer, Luisa;Gonzalez, Ana E. Aguilar;Weninger, Sophie T.;Atzmueller, Denise;Klasinc, Romana;Stanek, Gerold;Markowicz, Mateusz;Stockinger, Hannes;Stary, Georg
在皮肤蜱附着期间，食腔成为蜱唾液化合物和蜱传病原体的传播场所。然而，蜱虫喂养对人类皮肤的免疫学后果仍不清楚。在这里，我们评估了蜱叮咬后的人类皮肤和血液样本，并开发了一种模仿蓖麻硬蜱叮咬和蜱传病原体感染的人类皮肤外植体模型。蜱附着后，我们观察到快速发生的免疫调节模式，包括中性粒细胞和皮肤B和T细胞的增加。T细胞上调组织驻留标记物，而淋巴细胞细胞因子的产生受损。在伯氏疏螺旋体模型感染的早期阶段，我们检测到菌株特异性免疫反应和巨噬细胞和螺旋体之间的密切空间关系。用蜱唾液腺提取物预孵育螺旋体阻碍了免疫细胞的积累并增加了螺旋体的载量。总的来说，我们发现蜱虫喂养对皮肤免疫网络产生了深刻的变化，干扰了对蜱虫传播病原体的主要反应。
During cutaneous tick attachment, the feeding cavity becomes a site of transmission for tick salivary compounds and tick-borne pathogens. However, the immunological consequences of tick feeding for human skin remain unclear. Here, we assessed human skin and blood samples upon tick bite and developed a human skin explant model mimicking Ixodes ricinus bites and tick-borne pathogen infection. Following tick attachment, we observed rapidly occurring patterns of immunomodulation, including increases in neutrophils and cutaneous B and T cells. T cells upregulated tissue residency markers, while lymphocytic cytokine production was impaired. In early stages of Borrelia burgdorferi model infections, we detected strain-specific immune responses and close spatial relationships between macrophages and spirochetes. Preincubation of spirochetes with tick salivary gland extracts hampered accumulation of immune cells and increased spirochete loads. Collectively, we showed that tick feeding exerts profound changes on the skin immune network that interfere with the primary response against tick-borne pathogens.
影响因子：
4.6
作者：
Tomás-Cortázar J;Martín-Ruiz I;Barriales D;Pascual-Itoiz MÁ;de Juan VG;Caro-Maldonado A;Merino N;Marina A;Blanco FJ;Flores JM;Sutherland JD;Barrio R;Rojas A;Martínez-Chantar ML;Carracedo A;Simó C;García-Cañas V;Abecia L;Lavín JL;Aransay AM;Rodríguez H;Anguita J
通讯作者：
Anguita J