COMPARATIVE SENSITIVITY OF SHEEP AND CHICKEN EMBRYOS TO BLUETONGUE VIRUS AND OBSERVATIONS ON VIREMIA IN EXPERIMENTALLY INFECTED SHEEP
COMPARATIVE SENSITIVITY OF SHEEP AND CHICKEN EMBRYOS TO BLUETONGUE VIRUS AND OBSERVATIONS ON VIREMIA IN EXPERIMENTALLY INFECTED SHEEP
复制标题
DOI：
10.1111/j.1751-0813.1975.tb00053.x
复制
发表时间：
1975-01-01
影响因子：
1.1
通讯作者：
TADMOR, A
中科院分区：
文献类型：
--
作者：
GOLDSMIT, L;BARZILAI, E;TADMOR, A
在绵羊和鸡胚中检测了从现场疑似BT病例中收到的绵羊血液样品中的病毒滴度。这些感染的血液样本代表3种不同的BT病毒类型：4、10和16。进行了三次相同的实验，3种不同病毒类型各一次。制备感染血液样本的10倍稀释液，将每种血液稀释液1 ml静脉接种到一系列10至12月龄的易感绵羊中;同时将每种稀释液0.1 ml静脉接种到一系列10至13日龄的鸡胚中。在两种宿主系统中测定时，发现病毒滴度相似。接种病毒量与接种羊的症状严重程度之间无相关性。每天采集试验羊的血样，静脉接种CE检测病毒含量。从所有反应的绵羊中分离出病毒，有时早在PI 1天和迟至PI 30天检测到病毒。在临床体征发生前后的连续几天内，记录到log 10 4.0 - 7.0/ml血液的高滴度。接种的病毒量与病毒在血液中存留的天数似乎成反比。第22天血清样本的中和指数为3.5 - 4.5。在记录这些抗体水平的当天，从一些有反应的绵羊中分离出病毒。由于BT病毒在绵羊和CE中的比较同时滴定产生了类似的结果，因此提倡将CE的IV接种作为用于该疾病的实验室诊断的常规方法。
The virus titre in sheep blood samples received from BT-suspected cases in the field was assayed in sheep and in chicken embryos. These infected blood samples represented 3 different BT virus types: 4, 10 and 16. Three identical experiments were performed, one with each of the 3 different virus types. Ten-fold dilutions of the infected blood samples were prepared and 1 ml of each blood dilution was inoculated IV into series of 10 to 12-month old susceptible sheep; at the same time 0.1 ml of each dilution was inoculated IV into series of 10 to 13-day-old chicken embryos. The virus titre was found to be similar when assayed in the two host systems. There was no correlation between the amount of virus inoculated and the severity of symptoms in the inoculated sheep. The virus content in daily blood samples collected from the experimental sheep was assayed by IV inoculation of CE. Virus was isolated from all the reacting sheep and was detected sometimes as early as 1 day PI and as late as 30 days PI. A high titre of log10 4.0 to 7.0 per 1 ml of blood was recorded during several consecutive days before and after the onset of clinical signs. There seemed to be an inverse ratio between the amount of virus inoculated and the number of days the virus persisted in the bloodstream. The neutralisation index in day 22 serum samples was 3.5 to 4.5. Virus was isolated from some of the reacting sheep on the day that these antibody levels were recorded. Since the comparative simultaneous titrations of BT virus in sheep and in CE yielded similar results, the IV inoculation of CE is advocated as the routine method to be employed for laboratory diagnosis of this disease.