AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TAXOL AND TAXANES IN TAXUS SP TISSUES AND HUMAN PLASMA
AN ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF TAXOL AND TAXANES IN TAXUS SP TISSUES AND HUMAN PLASMA
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DOI：
10.1016/0022-1759(93)90253-4
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发表时间：
1993-01-14
影响因子：
2.2
通讯作者：
BYRNES, JB
中科院分区：
文献类型：
--
作者：
GROTHAUS, PG;RAYBOULD, TJG;BYRNES, JB
建立了一种快速、灵敏的间接竞争抑制酶免疫分析法（CIEIA）测定短叶红豆杉提取物中紫杉烷类化合物的含量。组织和人血浆中。制备了抗7-琥珀酰紫杉醇和匙孔血蓝蛋白结合物的高滴度兔多克隆抗体（pAb）。紫杉烷分析物的存在竞争性地抑制兔抗紫杉烷pAb与7-琥珀酰紫杉醇-牛血清白蛋白固相包被抗原的结合。CIEIA检测到紫杉醇和三尖杉宁碱的浓度低至0.3 ng/ml（3.5 × 10 - 4 μ M），但未检测到浆果赤霉素III或10-脱乙酰浆果赤霉素III的浓度低于1000 ng/ml（分别为1.7 μ M和1.8 μ M）。该方法可用于红豆杉中紫杉烷类化合物的含量测定。短叶提取物，并可用于监测紫杉醇治疗患者的紫杉醇血浆水平。
A rapid and sensitive indirect competitive inhibition enzyme immunoassay (CIEIA) method has been developed for quantitating taxanes in extracts of Taxus brevifolia Nutt. tissue and in human plasma. High titer rabbit polyclonal antibodies (pAb) were raised against a conjugate of 7-succinyltaxol and keyhole limpet hemocyanin. The presence of taxane analyte competitively inhibited the binding of the rabbit anti-taxane pAbs to a 7-succinyltaxol-bovine serum albumin solid phase coating antigen. The CIEIA detected taxol and cephalomannine in concentrations as low as 0.3 ng/ml (3.5 x 10(-4) muM), but did not detect baccatin III or 10-deacetylbaccatin III at concentrations below 1000 ng/ml (1.7 muM and 1.8 muM, respectively). This method is useful for estimating the taxane content of T. brevifolia extracts and may be useful for monitoring the taxol plasma level of taxol-treated patients.