Identification of small RNAs in late developmental stage of rice anthers
Identification of small RNAs in late developmental stage of rice anthers
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DOI：
10.1266/ggs.83.281
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发表时间：
2008-06-01
影响因子：
1.1
通讯作者：
Watanabe, Masao
中科院分区：
文献类型：
--
作者：
Fujioka, Tomoaki;Kaneko, Fumi;Watanabe, Masao
小RNA包括microRNA （miRNA）和小干扰RNA （siRNA）被认为是基因表达的抑制因子。有许多植物蛋白参与小RNA介导的基因沉默，如Dicer核糖核酸酶和RNA依赖的RNA聚合酶。然而，据报道，这些蛋白质中的大多数在植物雄性配子花粉发育后期不存在。为了阐明小rna在花粉成熟过程中的存在，我们从水稻花药（包括三细胞花粉）中克隆并测序了小rna。从56个候选小rna中，我们鉴定出2个已知的mirna (miR166和miR167)， 8个潜在的mirna和10个推测的异染色质sirna （hc- sirna）。RNA凝胶印迹分析清楚地表明，miR166和miR167在花药发育的单核花粉期积累，并一直持续到三细胞花粉期。我们对小RNA的克隆和RNA凝胶印迹分析使我们提出了小RNA介导的基因调控水稻雄性配子发育的可能功能。
Small RNAs including microRNA (miRNA) and small interfering RNA (siRNA) are known as repressors of gene expression. There are many plant proteins involved in small RNA-mediated gene silencing, such as Dicer ribonucleases and RNA-dependent RNA polymerases. However, most of these proteins have been reported to be absent in the late developmental stage of the plant male gamete, pollen. In order to clarify the existence of the small RNAs during maturation of pollen, we cloned and sequenced small RNAs from rice anthers including tricellular pollen. From fifty six candidates of small RNAs, we identified two known miRNAs (miR166 and miR167), eight potential miRNAs, and ten putative heterochromatic siRNAs (hc-siRNAs). RNA gel blot analyses clearly showed that miR166 and miR167 were accumulated in the uninuclear pollen stage of anther development and remained until the tricellular pollen stage. Our cloning and RNA gel blot analyses of small RNAs led us to propose a possible function of small RNA-mediated gene regulation for the development of male gametes in rice.