ADENINE NUCLEOTIDE METABOLISM AND ITS RELATION TO ORGAN VIABILITY IN HUMAN LIVER TRANSPLANTATION1
ADENINE NUCLEOTIDE METABOLISM AND ITS RELATION TO ORGAN VIABILITY IN HUMAN LIVER TRANSPLANTATION1
复制标题
人肝移植中腺嘌呤核苷酸代谢及其与器官活力的关系1
作者：
W. Kamiike;M. Burdelski;Prof. Dr. med. Gustav Steinhoff;B. Ringe;W. Lauchart;R. Pichlmayr
研究了人肝脏中腺嘌呤核苷酸代谢与缺血性损伤之间的关系。三十个移植的移植物根据其功能结果分为两组。通过高效液相色谱法测定细胞腺嘌呤核苷酸水平。冷缺血期间，三磷酸腺苷（ATP）水平与移植物功能不相关，但总腺嘌呤核苷酸（TAN）水平较低的两个移植物在移植后表现出较差的功能。再循环后，功能满意的移植物（n=24）中ATP水平恢复良好，为5.47±1.51 mUmol/g干重），但功能较差的移植物（n=6）中ATP水平仍然较低，为3.30pL（1.68 mUmol/g干重）（P<0.01）。胆汁产生量被用作初始功能的参数，在 24 个功能令人满意的移植物中，有 17 个在植入后不久就观察到了胆汁产生，但在 6 个功能较差的移植物中只有 1 个出现了胆汁产生。结论是，移植过程中腺胺核苷酸的损失和胆汁产生的缺乏是人肝移植中移植物受损的良好标志。
The relationship between adenine nucleotide metabolism and ischemic damage was studied in human liver. Thirty transplanted grafts were divided into two groups assording to their functional outcome. Cellular adenine nucleotide levels were assayed by high-performance liquid chromatography. During cold ischema, the adenosine triphosphate (ATP) level was not correlated with graft function, but two grafts with low total adenine nucleotides (TAN) levels showed poor function after transplantation. After recirculation, the ATP level showed good recovery in grafts that functioned satisfactorily (n=24), 5.47±1.51 mUmol/g dry weight), but remained low in poorly functioning grafts (n=6), 3.30pL 1.68 mUmol/g dry weight) (P<0.01). Bile production, used as a parameter of initial function, was observed shortly after implantation in 17 of 24 grafts that functioned satisfactorily, but in only 1 of 6 poorly functioning grafts. It is concluded that loss of ademine nucleotides and lack of bile production during transplantation are good markers of damaged grafts in human liver transplantation.