Genome-Wide Dissection of the Heat Shock Transcription Factor Family Genes in Arachis.
Genome-Wide Dissection of the Heat Shock Transcription Factor Family Genes in Arachis.
复制标题
花生热激转录因子家族基因的全基因组解析
DOI：
10.3389/fpls.2017.00106
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Wang P;Song H;Li C;Li P;Li A;Guan H;Hou L;Wang X
热激转录因子（Hsfs）是保护植物免受各种胁迫造成损害的重要转录因子（TFs）。野生花生全基因组序列的公布使对花生中Hsfs进行全基因组分析成为可能。在本研究中，分别从蔓花生（Arachis duranensis）和野生花生（A. ipaensis）中鉴定出16个和17个Hsf基因。我们在这两种花生中鉴定出16对直系同源Hsf基因对；然而，HsfXs仅在野生花生中被鉴定到。两种野生花生之间的直系同源对具有高度的同线性。基于系统发育关系，花生Hsfs被分为A、B和C组。选择压力分析表明，B组Hsf基因主要经历正选择，A组Hsfs受纯化选择影响。小规模的片段重复和串联重复可能在这些基因的进化中起重要作用。在大多数花生Hsf基因的启动子中发现了顺式作用元件，如ABRE、DRE和HSE。5个蔓花生Hsf基因和2个野生花生Hsf基因包含真菌诱导子响应元件，表明它们参与了对真菌感染的响应。这些基因在栽培花生受到非生物胁迫和黄曲霉感染时差异表达。接种黄曲霉后，AhHsf2和AhHsf14显著上调，表明它们可能在真菌抗性中起作用。
Heat shock transcription factors (Hsfs) are important transcription factors (TFs) in protecting plants from damages caused by various stresses. The released whole genome sequences of wild peanuts make it possible for genome-wide analysis of Hsfs in peanut. In this study, a total of 16 and 17 Hsf genes were identified from Arachis duranensis and A. ipaensis, respectively. We identified 16 orthologous Hsf gene pairs in both peanut species; however HsfXs was only identified from A. ipaensis. Orthologous pairs between two wild peanut species were highly syntenic. Based on phylogenetic relationship, peanut Hsfs were divided into groups A, B, and C. Selection pressure analysis showed that group B Hsf genes mainly underwent positive selection and group A Hsfs were affected by purifying selection. Small scale segmental and tandem duplication may play important roles in the evolution of these genes. Cis-elements, such as ABRE, DRE, and HSE, were found in the promoters of most Arachis Hsf genes. Five AdHsfs and two AiHsfs contained fungal elicitor responsive elements suggesting their involvement in response to fungi infection. These genes were differentially expressed in cultivated peanut under abiotic stress and Aspergillus flavus infection. AhHsf2 and AhHsf14 were significantly up-regulated after inoculation with A. flavus suggesting their possible role in fungal resistance.