Sub-millimeter endoscope demonstrates feasibility of in vivo reflectance imaging, fluorescence imaging, and cell collection in the fallopian tubes.
Sub-millimeter endoscope demonstrates feasibility of in vivo reflectance imaging, fluorescence imaging, and cell collection in the fallopian tubes.
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DOI：
10.1117/1.jbo.26.7.076001
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发表时间：
2021-07
影响因子：
3.5
通讯作者：
Barton JK
中科院分区：
文献类型：
--
作者：
Cordova R;Kiekens K;Burrell S;Drake W;Kmeid Z;Rice P;Rocha A;Diaz S;Yamada S;Yozwiak M;Nelson OL;Rodriguez GC;Heusinkveld J;Shih IM;Alberts DS;Barton JK
重要性：大多数高级别浆液性卵巢癌病例起源于输卵管上皮（FTE）中的浆液性输卵管上皮内癌（STIC）病变，使早期内镜检测成为可能。目的：细胞采集输卵管内窥镜（CAFE）的建立是为了满足定位潜在病理组织的要求，这些组织由自体荧光的改变或靶向荧光团的存在指示。包括用于从感兴趣区域进行细胞的定向刮取活检的通道。方法：分别使用标准分辨率目标和荧光标准测量成像分辨率和荧光灵敏度。在离体组织中测试原型，并对收集的细胞进行计数和处理。结果：测量的成像分辨率为5 mm距离，全视野为空气。捕获了离体猪生殖道中的反射率和荧光图像，并验证了通过人类生殖道的拟合。血细胞计数表明，在每刮的数量级上，可以从离体猪组织中收集活检。结论：符合FTE中查看STIC的所有要求，采集的细胞计数超过相关分析的输入要求。我们的实验室研究结果表明，在未来的临床试验中，CAFE设备用于体内成像和细胞收集的潜在效用。
Significance: Most cases of high-grade serous ovarian carcinoma originate as serous tubal intraepithelial carcinoma (STIC) lesions in the fallopian tube epithelium (FTE), enabling early endoscopic detection. Aim: The cell-acquiring fallopian endoscope (CAFE) was built to meet requirements for locating potentially pathological tissue indicated by an alteration in autofluorescence or presence of a targeted fluorophore. A channel was included for directed scrape biopsy of cells from regions of interest. Approach: Imaging resolution and fluorescence sensitivity were measured using a standard resolution target and fluorescence standards, respectively. A prototype was tested in ex vivo tissue, and collected cells were counted and processed. Results: Measured imaging resolution was at a 5-mm distance, and full field of view was in air. Reflectance and fluorescence images in ex vivo porcine reproductive tracts were captured, and fit through human tracts was verified. Hemocytometry counts showed that on the order of per scrape biopsy could be collected from ex vivo porcine tissue. Conclusions: All requirements for viewing STIC in the FTE were met, and collected cell counts exceeded input requirements for relevant analyses. Our benchtop findings suggest the potential utility of the CAFE device for in vivo imaging and cell collection in future clinical trials.