Sitosterol-β-glucoside as primer for cellulose synthesis in plants
Sitosterol-β-glucoside as primer for cellulose synthesis in plants
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DOI：
10.1126/science.1064281
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发表时间：
2002-01-04
期刊：
影响因子：
56.9
通讯作者：
Delmer, D
中科院分区：
文献类型：
--
作者：
Peng, LC;Kawagoe, Y;Delmer, D
植物中的纤维素合成需要β-1，4-葡聚糖链的起始、延伸和终止。链伸长的过程可能不同于链起始的过程。我们证明了CesA葡糖基转移酶通过使用谷甾醇-β-葡糖苷（SG）作为引物来启动葡聚糖聚合。棉纤维膜在也有利于纤维素合成的条件下从SG和尿苷5 '-二磷酸-葡萄糖（UDP-Glc）合成谷甾醇-纤维糊精（SCD）。由Korrigan（Kor）基因编码的纤维素酶是植物中纤维素合成所需的，其功能可以是从生长的聚合物链上切割SG。
Cellulose synthesis in plants requires beta -1,4-glucan chain initiation, elongation, and termination. The process of chain elongation is likely to be distinct from the process of chain initiation. We demonstrate that a CesA glucosyltransferase initiates glucan polymerization by using sitosterol-beta -glucoside (SG) as primer. Cotton fiber membranes synthesize sitosterol-cellodextrins (SCDs) from SG and uridine 5'-diphosphate-glucose (UDP-Glc) under conditions that also favor cellulose synthesis. The cellulase encoded by the Korrigan (Kor) gene, required for cellulose synthesis in plants, may function to cleave SG from the growing polymer chain.