Refinement of an open-microcavity optical biosensor for bacterial endotoxin test
Refinement of an open-microcavity optical biosensor for bacterial endotoxin test
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DOI：
10.1016/j.bios.2021.113436
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发表时间：
2021-06-20
影响因子：
12.6
通讯作者：
Ye, Jing Yong
中科院分区：
文献类型：
--
作者：
Akimov, Nikolay;Scudder, Jonathan;Ye, Jing Yong
鲎试剂（LAL）试验是一种广泛用于制药和生物技术行业检测细菌内毒素的体外试验。内毒素是革兰氏阴性菌细胞壁的结构成分，在体内具有严重的致病作用，可引起多器官系统功能障碍，增加死亡风险。为了满足由于药物和疫苗制造商需求增加而对LAL测定的日益增长的需求，我们开发了一种新的LAL测定方法。我们的检测机制与目前行业中使用的检测机制不同，并进行了改进，从而提高了检测灵敏度并缩短了检测时间。我们的研究利用开放式微腔光子晶体生物传感器来定量内毒素浓度。与商业标准方法相比，它已证明LAL测定灵敏度提高了10倍，并将测定所需的时间缩短了一半以上。此外，这种方法每次测试只需5 μ L鲎试剂，从而降低成本并保护鲎。本文报道的结果表明，使用光子晶体生物传感器的方法显着增强内毒素检测的可能性。
The Limulus Amebocyte Lysate (LAL) test is an in vitro assay widely used in the pharmaceutical and biotechnology industries to detect bacterial endotoxins. Endotoxin is a structural component of the cell wall of Gramnegative bacteria, which has serious pathogenic effects in the body and may cause dysfunction of multiple organ systems and increased risk of mortality. To address the growing need for LAL assays due to the increased demand from drug and vaccine manufacturers, we have developed a new LAL assay approach. Our detection mechanism is different and improved from those currently used in the industry, leading to increased test sensitivity and reduced assay time. Our study utilizes an open-microcavity photonic-crystal biosensor to quantify endotoxin concentrations. It has demonstrated an improved LAL assay sensitivity by 10 fold compared to the commercial standard methods and reduced the time needed for the assay by more than half. In addition, this approach requires as little as 5 mu L of LAL reagent per test, thereby decreasing costs and conserving horseshoe crabs. The results reported in this paper indicate the possibility of using the photonic-crystal biosensor based approach for significant enhancements of endotoxin testing.