Activation of Toll-like receptors 2, 3, and 4 on human melanoma cells induces inflammatory factors.
Activation of Toll-like receptors 2, 3, and 4 on human melanoma cells induces inflammatory factors.
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DOI：
10.1158/1535-7163.mct-08-0582
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发表时间：
2008-11
影响因子：
5.7
通讯作者：
Hoon DS
中科院分区：
文献类型：
--
作者：
Goto Y;Arigami T;Kitago M;Nguyen SL;Narita N;Ferrone S;Morton DL;Irie RF;Hoon DS
Toll样受体（TLR）已被证明在各种类型的癌症上表达;然而，其功能活性尚不清楚。我们检测了人类黑色素瘤细胞的TLR谱，发现TLR 2、TLR 3和TLR 4高表达。通过PCR阵列分析，TLR 2、TLR 3和TLR 4对黑色素瘤细胞的特异性刺激显示接头蛋白MyD 88以及下游信号转导因子核因子-κB和炎症反应相关因子的显著激活。TLR 2、TLR 3和TLR 4的特异性配体活化显示诱导细胞迁移。外周血淋巴细胞和黑色素瘤纯化的RNA显示激活黑色素瘤细胞上的TLR 3。这些研究显示了特异性TLR在黑素瘤细胞上的表达和功能活性，以及作为控制肿瘤进展的潜在治疗靶点。
Toll-like receptors (TLR) have been shown to be expressed on various types of cancers; however, their functional activity is not known. We examined TLR profiles of human melanoma cells and showed that TLR2, TLR3, and TLR4 were found to be highly expressed. By PCR array analysis, specific stimulation of TLR2, TLR3, and TLR4 on melanoma cells showed significant activation of the adaptor protein MyD88, as well as downstream signal transduction factors nuclear factor-κB and inflammatory response–related factors. Specific ligand activation of TLR2, TLR3, and TLR4 was shown to induce cell migration. Peripheral blood lymphocytes and melanoma purified RNA was shown to activate TLR3 on melanoma cells. These studies show expression and functional activity of specific TLRs on melanoma cells and as potential therapeutic targets to control tumor progression.