Targeting of hematopoietic progenitor cells with MR contrast agents
Targeting of hematopoietic progenitor cells with MR contrast agents
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DOI：
10.1148/radiol.2283020322
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发表时间：
2003-09-01
期刊：
影响因子：
19.7
通讯作者：
Link, TM
中科院分区：
文献类型：
--
作者：
Daldrup-Link, HE;Rudelius, M;Link, TM
目的：用不同的磁共振成像对比剂标记人造血祖细胞，获得造血祖细胞的1.5T磁共振图像。材料和方法：造血祖细胞用亚铁氰化物、阿罗莫司、磁性多糖纳米粒-转铁蛋白、P7228脂质体和加多喷妥双葡胺脂质体进行磁共振成像，序列分别为T1和T2加权自旋回波序列和快速场回波序列。通过测量标记细胞和非标记对照细胞的MR信号强度以及R1和R2*松弛速率来分析数据。通过Scheffe检验评估不同造影剂组的平均定量数据与对照细胞的显著差异。结果：所有造影剂在电子显微镜下均可见胞浆摄取，光谱学定量分析显示细胞内胞浆摄取。氧化铁标记的祖细胞与未标记的对照细胞相比，差异有统计学意义(P
PURPOSE: To label human hematopoietic progenitor cells with various magnetic resonance (MR) imaging contrast agents and to obtain 1.5-T MR images of them.MATERIALS AND METHODS: Hematopoietic progenitor cells, labeled with ferumoxides, ferumoxtran, magnetic polysaccharide nanoparticles-transferrin, P7228 liposomes, and gadopentetate dimeglumine liposomes underwent MR imaging with T1- and T2-weighted spin-echo and fast field-echo sequences. Data were analyzed by measuring MR signal intensities and R1 and R2* relaxation rates of labeled cells and nonlabeled control cells. Mean quantitative data for the various contrast agent groups were assessed for significant differences compared with control cells by means of the Scheffe test. As a standard of reference, MR imaging data were compared with electron microscopic and spectrometric data.RESULTS: For all contrast agents, intracellular cytoplasm uptake was demonstrated with electron microscopy and was quantified with spectrometry. When compared with nonlabeled control cells, progenitor cells labeled with iron oxides showed significantly (P