Shavenbaby couples patterning to epidermal cell shape control.
Shavenbaby couples patterning to epidermal cell shape control.
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DOI：
10.1371/journal.pbio.0040290
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发表时间：
2006-09
期刊：
影响因子：
9.8
通讯作者：
Plaza, Serge
中科院分区：
文献类型：
--
作者：
Chanut-Delalande, Helene;Fernandes, Isabelle;Roch, Fernando;Payre, Francois;Plaza, Serge
众所周知，发育程序在胚胎发生过程中起作用，决定动物的形态发生。然而，这些发育线索如何在形态发生过程中产生特定的细胞形状仍然是个谜。我们通过研究果蝇表皮的形态分化来解决这个问题，果蝇表皮的形态分化受到众所周知的调节回路的控制，导致平滑细胞和形成富含肌动蛋白的延伸(毛状体)的细胞的刻板模式。结果表明，转录因子ShavenBaby在毛状体的形成中起着关键作用，也是所有研究过的毛状体模式进化多样化的基础。为了深入了解形态分化的机制，我们试图确定shavenBaby的下游靶标。我们在这里展示了ShavenBaby通过转录激活不同类别的细胞效应器来控制表皮细胞的形状，直接促进肌动蛋白细丝的组织，调节细胞外基质，以及角质层的修饰。单独灭活shavenBaby的靶标会产生明显的毛状体缺陷，只有它们同时失活才能防止毛状体的形成。我们的数据显示，shavenBaby控制着一个进化上保守的发育模块，该模块由一组共同负责毛状体形成的基因组成，这为研究形态发生过程中的分子机制以及它们如何影响动物形态的进化提供了新的线索。作者探索了ShavenBaby(SVB)如何协调专业的富含肌动蛋白的结构的形成，称为毛状体，这些结构构成了背毛和腹侧牙的独特形态。
It is well established that developmental programs act during embryogenesis to determine animal morphogenesis. How these developmental cues produce specific cell shape during morphogenesis, however, has remained elusive. We addressed this question by studying the morphological differentiation of the Drosophila epidermis, governed by a well-known circuit of regulators leading to a stereotyped pattern of smooth cells and cells forming actin-rich extensions (trichomes). It was shown that the transcription factor Shavenbaby plays a pivotal role in the formation of trichomes and underlies all examined cases of the evolutionary diversification of their pattern. To gain insight into the mechanisms of morphological differentiation, we sought to identify shavenbaby's downstream targets. We show here that Shavenbaby controls epidermal cell shape, through the transcriptional activation of different classes of cellular effectors, directly contributing to the organization of actin filaments, regulation of the extracellular matrix, and modification of the cuticle. Individual inactivation of shavenbaby's targets produces distinct trichome defects and only their simultaneous inactivation prevent trichome formation. Our data show that shavenbaby governs an evolutionarily conserved developmental module consisting of a set of genes collectively responsible for trichome formation, shedding new light on molecular mechanisms acting during morphogenesis and the way they can influence evolution of animal forms. The authors explore how Shavenbaby (Svb) orchestrates the formation of specialized actin-rich structures, called trichomes, that underlie the distinctive morphology of dorsal hairs and ventral denticles.