A new dimension in suspension fusion techniques with polyethylene glycol
A new dimension in suspension fusion techniques with polyethylene glycol
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聚乙二醇悬浮融合技术的新维度
DOI：
10.1007/bf01542972
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发表时间：
1977
期刊：
影响因子：
--
通讯作者：
R. Davidson
中科院分区：
文献类型：
--
作者：
Kathleen A. O'Malley;R. Davidson
当细胞在处理过程中附着于基底时，聚乙二醇（PEG）以比当细胞在悬浮液中处理时高得多的频率诱导哺乳动物细胞的杂交。由于许多细胞类型，例如，由于淋巴细胞不能附着在基质上，因此开发了一种用于PEG诱导的悬浮细胞融合的新技术。该技术被称为“薄饼融合”，其基于将悬浮细胞离心到盖玻片上，并将盖玻片上的细胞进行PEG处理，就好像它们附着在基底上一样。利用该技术，可以大大提高不能附着于基质的人白色血细胞的杂交频率。
Polyethylene glycol (PEG) induces the hybridization of mammalian cells at a much higher frequency when the cells are attached to a substrate during treatment than when the cells are treated in suspension. Since many cell types, e.g., lymphocytes, cannot attach to a substrate, a new technique for the PEG-induced fusion of cells in suspension was developed. This technique, referred to as “pancake fusion,” is based on the centrifugation of suspended cells onto a coverslip and the PEG treatment of the cells on the coverslip as if they were attached to a substrate. With this technique, the frequency of hybridization of human white blood cells, which are incapable of attaching to a substrate, can be greatly increased.