Fabrication of a novel laccase biosensor based on silica nanoparticles modified with phytic acid for sensitive detection of dopamine
Fabrication of a novel laccase biosensor based on silica nanoparticles modified with phytic acid for sensitive detection of dopamine
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DOI：
10.1016/j.snb.2014.03.002
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发表时间：
2014-07-01
影响因子：
8.4
通讯作者：
Huang, Xiaohua
中科院分区：
文献类型：
--
作者：
Wang, Kuai;Liu, Pengcheng;Huang, Xiaohua
用于生物物种超灵敏检测的新型纳米材料的开发受到了极大的关注。在此，我们报告的植酸功能化的二氧化硅纳米粒子（SiO2-PA NPs）的设计和合成及其生物医学传感器的应用，灵敏地检测多巴胺。通过透射电子显微镜（TEM）和X射线能谱仪（EDS）对SiO2-PA纳米粒子进行表征。利用圆二色光谱（CD）研究了SiO2-PA纳米粒子与漆酶的生物相容性。此外，制备了基于SiO2-PA纳米粒子的漆酶生物传感器，并研究了该漆酶生物传感器的电化学性能。结果表明，该生物传感器对DA具有良好的电催化活性，线性范围宽（0.99-103.10 μ M），检测限低（0.26 ± 0.003 μ M）。这可能是由于SiO2-PA纳米粒子具有良好的生物相容性和纳米效应。该传感器成功地用于药物和兔血清样品中DA的测定。仿生材料科学的发展为物质检测提供了一个新的应用平台。(C)2014爱思唯尔有限公司版权所有。
The development of novel nanomaterials for the ultra-sensitive detection of biological species has received great attention. Herein we report the design and synthesis of phytic acid functionalized silica nanoparticles (SiO2-PA NPs) and their biomedical sensor application for sensitive detection of dopamine. The SiO2-PA NPs were characterized by transmission electron microscopy (TEM) and Energy dispersive X-ray spectrometer (EDS). The biocompatibility between SiO2-PA NPs and laccase was also investigated by circular dichroism (CD) spectra. Moreover, the novel laccase biosensor based on SiO2-PA NPs for sensitive detection of dopamine (DA) was fabricated, and the electrochemical properties of this laccase biosensor were investigated. The results showed the biosensor had good electrocatalytic activity toward DA with a wide linear range (0.99-103.10 mu M) and a low detection limit 0.26 +/- 0.003 mu M. It can be attributed to the good biocompatibility and nano-effect of SiO2-PA NPs. The biosensor was successfully tested for determination of DA in pharmaceutical and rabbit blood serum samples. The development of biomimetic materials science will offer a novel platform for application to substance detection. (C) 2014 Elsevier B.V. All rights reserved.