Isolation of Koala Retroviruses from Koalas in Japan
Isolation of Koala Retroviruses from Koalas in Japan
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DOI：
10.1292/jvms.10-0250
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发表时间：
2011-01-01
影响因子：
1.2
通讯作者：
Ohata, Takuji
中科院分区：
文献类型：
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作者：
Miyazawa, Takayuki;Shojima, Takayuki;Ohata, Takuji
考拉逆转录病毒（KoRV）被认为与考拉的白血病、淋巴瘤和免疫缺陷样疾病有关。因此，我们对神户市王子动物园的5只昆士兰考拉进行了KoRV感染的试点研究。用聚合酶链反应检测全血和粪便基因组DNA中KoRV的部分env和pol基因，结果5只考拉均呈KoRV原病毒阳性。在重组人白细胞介素-2的存在下，我们成功地从不到1ml的血液中培养了14天以上的考拉淋巴细胞。通过将淋巴细胞与人胚胎肾（HEK） 293T细胞共培养，我们从5只考拉身上分离到了korv。我们将这些分离株命名为OJ-1至OJ-5。电镜下观察到慢性KoRV OJ-4株感染的HEK 293T细胞中存在c型逆转录病毒颗粒。这是日本动物园首次从考拉身上分离出KoRV。
Koala retrovirus (KoRV) is considered to be associated with leukemia, lymphoma and immunodeficiency-like diseases in koalas. We therefore conducted a pilot study of KoRV infection in five Queensland koalas in Kobe Municipal Oji Zoo. By polymerase chain reaction to detect partial env and pol genes of KoRV in genomic DNA isolated from whole blood and feces, all five koalas were found to be positive for KoRV proviruses. We succeeded in culturing koala lymphocytes from less than 1 ml blood for over 14 days in the presence of recombinant human interleukin-2. By coculturing the lymphocytes with human embryonic kidney (HEK) 293T cells, we isolated KoRVs from all five koalas. We designated these isolates as strains OJ-1 to OJ-5. By electron microscopy, we observed C-type retroviral particles in HEK 293T cells chronically infected with KoRV strain OJ-4. This is the first report on the isolation of KoRV from koalas in a Japanese zoo.