A role for apoptosis in the pathogenesis of AIDS-related idiopathic esophageal ulcers.
A role for apoptosis in the pathogenesis of AIDS-related idiopathic esophageal ulcers.
复制标题
细胞凋亡在艾滋病相关特发性食管溃疡发病机制中的作用。
DOI：
10.1086/593671
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Small,MB
中科院分区：
文献类型：
--
作者：
Houghton,JM;Korah,RM;Kim,KH;Small,MB
缺乏对特发性食管溃疡（IEU）相关组织破坏机制的了解，给临床医生带来了诊断和治疗的困境。凋亡在IEU中的可能作用，如内窥镜和组织学观察所示，通过原位缺口末端标记（TUNEL）检查存档组织中的凋亡相关DNA片段来研究。在紧邻IEU的粘膜细胞中观察到高水平的凋亡。正常对照组织中几乎不存在凋亡细胞，而人类免疫缺陷病毒（HIV）感染患者IEU中病变和脱落组织的边缘和基底显示凋亡细胞死亡水平升高。然而，已知病因的食管溃疡患者的组织样本显示粘膜细胞无凋亡。这些数据支持细胞凋亡在IEU发病机制中的作用，并提示了一种涉及HIV相关旁观者杀死未感染粘膜细胞的机制。
Lack of understanding of the mechanism of tissue destruction associated with idiopathic esophageal ulcers (IEUs) poses a diagnostic and therapeutic dilemma for the clinician. The possible role of apoptosis in IEUs, as suggested by endoscopic and histologic observations, was investigated by examination of archival tissues for apoptosis-related DNA fragmentation using in situ nick end labeling (TUNEL). High levels of apoptosis were observed in mucosal cells immediately adjacent to IEUs. Apoptotic cells were virtually absent in normal control tissues, while the edges and bases of lesions and sloughed-off tissues in IEUs in human immunodeficiency virus (HIV)—infected patients showed elevated levels of apoptotic cell death. However, tissue samples from patients with esophageal ulcerations of known etiology showed no apoptosis of mucosal cells. These data support a role for apoptosis in the pathogenesis of IEUs and suggest a mechanism involving HIV-associated bystander killing of uninfected mucosal cells.