Isolation and characterization of a mutant of Staphylococcus aureus deficient in autolytic activity
Isolation and characterization of a mutant of Staphylococcus aureus deficient in autolytic activity
复制标题
缺乏自溶活性的金黄色葡萄球菌突变体的分离和表征
DOI：
10.1128/jb.125.3.961-967.1976
复制
发表时间：
1976
影响因子：
3.2
通讯作者：
R. Gilpin
中科院分区：
文献类型：
--
作者：
A. Chatterjee;W. Wong;F. Young;R. Gilpin
A mutant of Staphylococcus aureus H (RUS3) uas isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. The rate of autolysis of whole cells and isolated cell walls of RUS3 was less than 10% of the parent strain. In addition, the ability of the crude soluble enzyme isolated from RUS3 to degrade cell walls was negligible compared with the parent strain. The cell wall composition and the generation time of RUS3 were comparable to the parent strain. Unlike S. aureus H, RUS3 grew in clumps and did not undergo cell wall turnover. Both strains exhibited identical kinetics of killing by penicillin G. This may indicate that autolytic enzymes play a role in cell wall turnover and cell separation, but in S. aureus most of the autolytic activity is unrelated to the lethal effect of cell wall antibiotics.
