Cellular radiosensitivity of a rat brain tumor
Cellular radiosensitivity of a rat brain tumor
复制标题
大鼠脑肿瘤的细胞放射敏感性
作者：
J. Leith;Wolfgang A. Schilling;K. Wheeler
研究了大鼠模型脑肿瘤系统对单剂量x射线照射的反应。实体瘤在原位暴露，移除，分离成单细胞，并在组织培养中生长。在麻醉、呼吸空气的大鼠和在氮气窒息照射前5分钟死亡的大鼠的肿瘤中，克隆生成细胞的存活比例是x射线剂量水平的函数。空气呼吸大鼠肿瘤辐照后生存曲线参数为：DO = 295 rad；Dq = 350 rad；n = 3.0。同样，氮气窒息大鼠肿瘤辐照后的生存参数为：DO = 625 rad；Dq = 675拉德；n = 3.0。体外组织培养时，这些肿瘤细胞的存活曲线只有如下参数：DO = 225 rad；Dq = 350 rad；n = 4.7。从体内到体外肿瘤细胞存活曲线的数据表明，在该脑肿瘤中没有明显的缺氧部分。
The response of a model brain tumor system in the rat to single doses of x irradiation has been studied. Solid tumors were exposed in situ, removed, dissociated into single cells, and grown in tissue culture. The fraction of surviving clonogenic cells was determined as a function of x‐ray dose level for tumors irradiated in anesthetized, air‐breathing rats, and for tumors irradiated in rats killed 5 minutes prior to irradiation by nitrogen gas asphyxiation. The parameters of the survival curve for tumors irradiated in air‐breathing rats were: DO = 295 rads; Dq = 350 rads; and n = 3.0. Similarly, the survival parameters for tumors irradiated in nitrogen gas asphyxiated rats were: DO = 625 rads; Dq = 675 rads; and n = 3.0. The survival curve of these tumor cells when assayed in in vitro tissue culture only has parameters of: DO = 225 rads; Dq = 350 rads; and n = 4.7. Data from the in vivo to in vitro tumor cell survival curves suggest that there is no significant hypoxic fraction in this brain tumor.