Increased miR-222 in H. pylori-associated gastric cancer correlated with tumor progression by promoting cancer cell proliferation and targeting RECK
Increased miR-222 in H. pylori-associated gastric cancer correlated with tumor progression by promoting cancer cell proliferation and targeting RECK
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幽门螺杆菌相关胃癌中 miR-222 的增加通过促进癌细胞增殖和靶向 RECK 与肿瘤进展相关
DOI：
10.1016/j.febslet.2012.01.025
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发表时间：
2012-03-23
期刊：
影响因子：
3.5
通讯作者：
Mao, Xu-Hu
中科院分区：
文献类型：
--
作者：
Li, Na;Tang, Bin;Mao, Xu-Hu
关于 microRNA (miRNA) 在幽门螺杆菌 (H. pylori) 诱导的胃癌发生过程中的潜在作用知之甚少。在这里，我们发现 microRNA-222 (miR-222) 在幽门螺杆菌感染的胃粘膜和胃癌中表达上调。 miR-222 的异位表达促进体外细胞增殖和集落形成。从机制上讲，我们将 RECK 确定为 miR-222 的新靶标，并通过体外 mRNA 表达的负相关性证实了它们之间的关系。此外，我们发现 RECK 的 RNA 干扰沉默可以模拟 miR-222 的致癌作用。总的来说，幽门螺杆菌可能通过上调miR-222在癌发生过程中发挥启动作用，miR-222通过促进增殖和抑制RECK进一步参与癌症的进展。 (C) 2012 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Little is known about the potential role of microRNAs (miRNAs) in the carcinogenesis of gastric cancer induced by Helicobacter pylori (H. pylori). Here, we showed that microRNA-222 (miR-222) was up-regulated in H. pylori-infected gastric mucosa and gastric cancer. Ectopic expression of miR-222 promoted cell proliferation and colony formation in vitro. Mechanistically, we identified RECK as a novel target of miR-222, and also confirmed their relationship by the inverse correlation of mRNA expression ex vivo. Furthermore, we found that RNA interference silencing of RECK can mimic the oncogenic effects of miR-222. Collectively, H. pylori may function as an initiator in the process of carcinogenesis by up-regulating miR-222, which further participates in the progression of cancer by promoting proliferation and inhibiting RECK. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.