Drosophila Stem Cells Share a Common Requirement for the Histone H2B Ubiquitin Protease Scrawny
Drosophila Stem Cells Share a Common Requirement for the Histone H2B Ubiquitin Protease Scrawny
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DOI：
10.1126/science.1165678
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发表时间：
2009-01-09
期刊：
影响因子：
56.9
通讯作者：
Spradling, Allan C.
中科院分区：
文献类型：
--
作者：
Buszczak, Michael;Paterno, Shelley;Spradling, Allan C.
不同组织内的干细胞都需要促进自我更新的染色质构型，但已知很少有染色质蛋白质调节多种类型的干细胞。我们描述了一个果蝇基因，scrawny（scny），编码一个泛素特异性蛋白酶，这是所需的生殖细胞，上皮细胞和肠干细胞。像它的酵母相对UBP 10，瘦去泛素化组蛋白H2B和基因沉默的功能。与先前对这种染色质调控保守途径的研究一致，scny突变细胞具有升高水平的泛素化H2B和三甲基化H3K4。我们的研究结果表明，通过scny抑制H2B泛素化是干细胞内的一种常见机制，用于抑制关键分化基因（包括Notch靶基因）的过早表达。
Stem cells within diverse tissues share the need for a chromatin configuration that promotes self- renewal, yet few chromatin proteins are known to regulate multiple types of stem cells. We describe a Drosophila gene, scrawny ( scny), encoding a ubiquitin- specific protease, which is required in germline, epithelial, and intestinal stem cells. Like its yeast relative UBP10, Scrawny deubiquitylates histone H2B and functions in gene silencing. Consistent with previous studies of this conserved pathway of chromatin regulation, scny mutant cells have elevated levels of ubiquitinylated H2B and trimethylated H3K4. Our findings suggest that inhibiting H2B ubiquitylation through scny represents a common mechanism within stem cells that is used to repress the premature expression of key differentiation genes, including Notch target genes.