T-cell responses to tyrosinase-derived self-peptides in patients with leukoderma induced by rhododendrol: Implications for immunotherapy targeting melanoma
T-cell responses to tyrosinase-derived self-peptides in patients with leukoderma induced by rhododendrol: Implications for immunotherapy targeting melanoma
复制标题
杜鹃醇诱导的白皮病患者 T 细胞对酪氨酸酶衍生自肽的反应：对针对黑色素瘤的免疫治疗的影响
DOI：
10.1159/000441217
复制
发表时间：
2016
期刊：
影响因子：
3.4
通讯作者：
Matsushita S.
中科院分区：
文献类型：
--
作者：
Takagi R.;Kawano M.;Nakamura K.;Tsuchida T.;Matsushita S.
背景杜鹃醇是美白化妆品中的一种酚类化合物，可结合并抑制酪氨酸酶，在日本有报道可诱导白血病。只有2%的化妆品用户受到影响，他克莫司是有效的治疗的conditions. ObjectiveTest的假设，这种疾病是一种自身免疫disorder.MethodsShort-term的T细胞系建立使用外周血单个核细胞从8例人黑色素瘤相关和酪氨酸酶衍生的合成肽。杜鹃醇对黑色素瘤免疫的影响也examined.Results7的8例患者为HLA-DR 4阳性。观察到I类和II类限制性和酪氨酸酶肽特异性T细胞应答。免疫小鼠与杜鹃醇处理和辐射B16黑色素瘤细胞成功地延迟了黑色素瘤细胞的生长在vivo. ConclusionRhodododendrol诱导白血病是一种自身免疫性疾病，与杜鹃醇作为一个环境因素和HLA-DR 4作为一个遗传因素。杜鹃醇可能对治疗黑色素瘤有效。
BackgroundRhododendrol, a phenolic compound contained in lightening/whitening cosmetics, can bind and inhibit tyrosinase and was reported to induce leukoderma in Japan. Only 2% of the cosmetics users are affected, and tacrolimus is effective in treatment of the condition.ObjectiveTo test the hypothesis that the disease is an autoimmune disorder.MethodsShort-term T-cell lines were established using peripheral blood mononuclear cells from 8 patients with human melanoma-associated and tyrosinase-derived synthetic peptides. The effects of rhododendrol on melanoma immunization were also examined.ResultsSeven out of 8 patients were positive for HLA-DR4. Both class I-and class II-restricted and tyrosinase peptide-specific T-cell responses were observed. Immunization of mice with rhododendrol-treated and irradiated B16 melanoma cells successfully delayed the growth of melanoma cells in vivo.ConclusionRhododendrol-induced leukoderma is an autoimmune disorder, with rhododendrol as an environmental factor and HLA-DR4 as a genetic factor. Rhododendrol might be effective in treating melanomas.