Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration.
Role of oxidants in ischemia/reperfusion-induced granulocyte infiltration.
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DOI：
10.1152/ajpgi.1990.258.2.g185
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发表时间：
1990-02
期刊：
影响因子：
--
通讯作者：
B. Zimmerman;M. Grisham;D. Granger
中科院分区：
文献类型：
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作者：
B. Zimmerman;M. Grisham;D. Granger
本研究的目的是确定是否过氧化氢，铁，和/或羟基自由基发挥作用，在缺血/再灌注（I/R）诱导的粒细胞浸润在猫小肠和是否形成化学引诱物时，超氧化物或过氧化氢与猫细胞外液反应。在体内测定的粒细胞浸润的组织髓过氧化物酶活性的测量，无论是在肠粘膜（I/R研究）或真皮（趋化性研究），而在体外测量的粒细胞迁移获得使用Boyden室。用过氧化氢酶或铁螯合剂去铁胺治疗显著减弱了缺血肠再灌注引起的粒细胞浸润到粘膜中。两个羟基自由基清除剂，二甲基亚砜（DMSO）和二甲基硫脲（DMTU），也评价了他们的能力，调节I/R诱导的粒细胞浸润。DMTU显著减弱I/R诱导的粒细胞聚集，而DMSO没有影响。在其他实验中，我们无法刺激粒细胞迁移与猫血浆暴露于超氧化物生成系统使用在体外和体内模型的白细胞趋化性。然而，在亚铁或血红蛋白存在下的过氧化氢确实显著增加猫血浆的趋化活性。从我们的研究中获得的结果表明，无论是过氧化氢或自由基物种来自超氧化物和过氧化氢与铁的相互作用引起I/R诱导的粒细胞浸润在肠道中。
The objective of this study was to determine whether hydrogen peroxide, iron, and/or hydroxyl radicals play a role in ischemia/reperfusion (I/R)-induced granulocyte infiltration in the feline small intestine and whether a chemoattractant is formed when superoxide or hydrogen peroxide reacts with feline extracellular fluid. In vivo determinations of granulocyte infiltration consisted of measurements of tissue myeloperoxidase activity in either the intestinal mucosa (I/R studies) or dermis (chemotaxis studies), whereas in vitro measurements of granulocyte migration were obtained using a Boyden chamber. Treatment with either catalase or the iron chelator deferoxamine significantly attenuated granulocyte infiltration into the mucosa induced by reperfusion of the ischemic intestine. Two hydroxyl radical scavengers, dimethyl sulfoxide (DMSO) and dimethylthiourea (DMTU), were also evaluated for their ability to modulate I/R-induced granulocyte infiltration. DMTU significantly attenuated the I/R-induced granulocyte accumulation, whereas DMSO had no effect. In other experiments, we were unable to stimulate granulocyte migration with feline plasma exposed to superoxide-generating systems using both in vitro and in vivo models of leukocyte chemotaxis. However, hydrogen peroxide in the presence of either ferrous iron or hemoglobin did significantly increase the chemotactic activity of cat plasma. The results obtained from our studies suggest that either hydrogen peroxide or radical species derived from the interaction of superoxide and hydrogen peroxide with iron elicit I/R-induced granulocyte infiltration in the intestine.