Expression Profiling of Circular RNAs and Micrornas in Heart Tissue of Mice with Alcoholic Cardiomyopathy.
Expression Profiling of Circular RNAs and Micrornas in Heart Tissue of Mice with Alcoholic Cardiomyopathy.
复制标题
酒精性心肌病小鼠心脏组织中环状 RNA 和 Micrornas 的表达谱。
DOI：
10.1159/000489596
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发表时间：
2018
影响因子：
--
通讯作者：
Qian Jinqiao
中科院分区：
文献类型：
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作者：
Yang Yuqiao;Chen Hongmei;Ding Nina;Wang Shuo;Duan Zhantao;Birnbaum Yochai;Ye Yumei;Qian Jinqiao
背景/目的长期大量饮酒可能导致酒精性心肌病。本研究旨在筛选酒精性心肌病小鼠心脏组织中差异表达的microRNAs和环状RNA，以揭示其分子机制。方法在建立小鼠酒精性心肌病模型的基础上，采用高通量芯片技术筛选酒精处理组和对照组三个心脏组织中差异表达的microRNAs和环状RNA。我们利用生物信息学软件对与酒精性心肌病密切相关的差异表达的非编码RNA的功能和生物信号通路进行了分析，以确定一些与酒精性心肌病密切相关的mRNAs及其生物信号通路。结果与对照组相比，酒精性心肌病组有19个microRNAs和265个环状RNA有差异表达。通过生物信息学软件对基因功能和信号通路进行分析，发现差异表达的mRNAs与糖代谢相关。结论慢性饮酒可改变心肌组织非编码RNA谱，与酒精性心肌病的发病机制密切相关。
Background/AimsChronic heavy alcohol consumption may result in alcoholic cardiomyopathy. This study was designed to screen differentially expressed microRNAs and circular RNAs in heart tissue of mice with alcoholic cardiomyopathy to reveal the underlying molecular mechanism.MethodsHaving established a murine alcoholic cardiomyopathy model, we screened differentially expressed microRNAs and circular RNAs in three heart samples from the alcohol-treated and control groups by high-throughput microarray analysis. We analyzed the function and biological signaling pathways of differentially expressed non-coding RNAs closely related to alcoholic cardiomyopathy using bioinformatics software to identify some mRNAs and their biological signaling pathways closely related to alcoholic cardiomyopathy.ResultsNineteen microRNAs and 265 circular RNAs were differentially expressed in the alcohol-treated group compared with the control group. After analyzing gene function and signaling pathways by bioinformatics software, we found that the differentially expressed mRNAs were associated with carbohydrate metabolism.ConclusionsChronic alcohol consumption can change the non-coding RNA profile of heart tissue, which is closely related to the pathological mechanisms of alcoholic cardiomyopathy.