Effect of metronidazole and modulation of cytokine production on human periodontal ligament cells
Effect of metronidazole and modulation of cytokine production on human periodontal ligament cells
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DOI：
10.1016/j.intimp.2010.04.004
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发表时间：
2010-07-01
影响因子：
5.6
通讯作者：
Tufano, Maria Antonietta
中科院分区：
文献类型：
--
作者：
Rizzo, Antonietta;Paolillo, Rossella;Tufano, Maria Antonietta
牙周炎是一种多因素的多微生物感染，其特征是破坏性的炎症过程，影响牙齿支持组织，导致牙周袋形成，牙槽骨吸收，最终导致牙齿脱落。牙周病原体及其毒力因子如脂多糖（LPS）对宿主免疫细胞和常驻细胞的持续攻击导致细胞因子分泌增强和不受控制。后者直接或间接参与组织破坏和骨吸收。甲硝唑（MTZ）是一种广泛使用的抗菌药物。抗生素的免疫调节作用可能影响人牙周膜细胞对感染的局部反应程度。HPLC在口腔的免疫应答中发挥作用。此外，HPLC可以产生细胞因子，增加炎症反应和正常通信的供应。MTZ也被提出用于牙周治疗领域，无论是全身给药还是局部可生物降解的缓释剂。凝胶形式的MTZ局部给药显著降低了全身副作用。本研究的目的，是模拟在患病牙周部位发生的体内条件，并评估MTZ对分离的HPLCs的活力的影响。还评价了MTZ调节HPLC中白细胞介素（IL）-1 β、IL-6、IL-8、IL-12和肿瘤坏死因子α（TNF-α）释放的能力，无论是否用牙龈卟啉单胞菌的LPS处理。结果表明，MTZ对HPLC无细胞毒性作用，并能抑制所分析的促炎细胞因子的产生。MTZ确定免疫调节作用的能力可以在牙周研究领域提供可能的治疗应用。(C)2010 Elsevier B. V.保留所有权利。
Periodontitis is a multifactorial polymicrobial infection characterized by a destructive inflammatory process affecting tooth-supporting tissues and resulting in periodontal pocket formation, alveolar bone resorption and, eventually, tooth loss. The continuous challenge of host immune and resident cells by periodontopathogens and their virulence factors, such as lipopolysaccharide (LPS), results in enhanced and uncontrolled secretion of cytokines. The latter directly or indirectly participate in tissue destruction and bone resorption. Metronidazole (MTZ) is a widely used antimicrobial agent. The immunomodulatory effects of antibiotics might influence the degree of the local response to infection on the human periodontal ligament cell (HPLC). HPLCs play a role in the immune response of the oral cavity. In addition, HPLC can produce cytokines that increase the inflammatory response and that supply for normal communication. MTZ has also been proposed in the field of periodontal therapy either with a systemic administration or with local biodegradable sustained-release agents. The local administration of MTZ in the form of gel significantly reduces the systemic side effects. The aim of the present study, was to simulate the in vivo conditions occurring in diseased periodontal sites, and to evaluate the effects of MTZ on the viability of isolated HPLCs. The ability of MTZ to modulate the release of interleukin (IL)-1 beta, IL-6, IL-8, IL-12 and tumor necrosis factor alpha (TNF-alpha) in HPLC, treated or not with LPS of Porphyromonas gingivalis was also evaluated. The results obtained showed that MTZ had no cytotoxic effect on HPLC and was able to inhibit the production of pro-inflammatory cytokines analyzed. The ability of MTZ to determine immunomodulatory effects could provide possible therapeutic applications in the field of periodontal research. (C) 2010 Elsevier B.V. All rights reserved.