Immobilization stress may increase plasma interleukin-6 via central and peripheral catecholamines.
Immobilization stress may increase plasma interleukin-6 via central and peripheral catecholamines.
复制标题
固定应激可能通过中枢和外周儿茶酚胺增加血浆白介素 6。
作者：
A. Takaki;Qin;A. Somogyvári;A. Arimura
最近有报道称，身体和心理应激均会升高血浆白细胞介素（IL）-6水平，而与内毒素血症、组织损伤或炎症无关。然而，这些模型中血浆IL-6升高的机制知之甚少。在本研究中，使用IL-6依赖性小鼠杂交瘤亚克隆B 9细胞系测量血浆IL-6水平，该细胞系通常由其他研究者使用。我们首先证明，制动（IM）的压力，一个典型的生理心理应激，增加血浆IL-6水平。下丘脑-垂体-肾上腺（HPA）轴和中枢及外周儿茶酚胺能系统在IM诱导的血浆IL-6升高中的作用进行了研究，因为这些机制在宿主防御应激中起重要作用。在IM之前、期间和之后通过留置颈静脉导管采集血样;从每只动物连续采集的样本数量为12-13。将血细胞重悬于盐水溶液中，并在每次采血后通过同一导管注射至动物体内，以防止血容量损失。在开始束缚后，在固定30或120 min的动物中，血浆IL-6水平在60 min时显著升高，并在90 min时达到峰值。（1，905 +/-414 U/ml）显著高于接受30 min IM的动物（837 +/-95 U/ml; p < 0.05）。（250字处删节）
It has recently been reported that both physical and psychological stress elevate plasma interleukin (IL)-6 levels independently of endotoxemia, tissue damage, or inflammation. However, the mechanism of plasma IL-6 elevation in these models is poorly understood. In the present study, plasma IL-6 levels were measured using the IL-6-dependent murine hybridoma subclone B9 cell line, which is commonly used by other investigators. We first demonstrated that an immobilization (IM) stress, a typical physicopsychological stress, increased plasma IL-6 levels. Then the contribution of the hypothalamic-pituitary-adrenal (HPA) axis and the central and peripheral catecholaminergic systems in IM-induced plasma IL-6 elevation were examined because these mechanisms play important roles in host defense against stress. Blood samples were collected through an indwelling jugular venous catheter before, during, and after IM; the number of samples taken serially from each animal was 12-13. Blood cells were resuspended in a saline solution and injected into the animals through the same catheter after each blood collection in order to prevent loss of blood volume. After initiation of restraint, plasma IL-6 levels significantly increased at 60 min and peaked at 90 min in the animals immobilized for either 30 or 120 min. The peak levels of IM-induced plasma IL-6 in the animals immobilized for 120 min (1,905 +/- 414 U/ml) were significantly higher than those in the animals subjected to 30 min IM (837 +/- 95 U/ml; p < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)
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影响因子：
5.1
作者：
SULLIVAN, JS;KILPATRICK, L;HARRIS, MC
通讯作者：
HARRIS, MC
大鼠中响应 IL-6 分泌 ACTH 的核心机制涉及：与 IL-1 β 的比较和相互作用。
影响因子：
4.1
作者：
Matta,SG;Weatherbee,J;Sharp,BM
通讯作者：
Sharp,BM
垂体前叶细胞在体外产生白细胞介素 6。
影响因子：
4.8
作者：
Spangelo,BL;MacLeod,RM;Isakson,PC
通讯作者：
Isakson,PC
DOI：
10.1073/pnas.87.19.7512
发表时间：
1990-10-01
影响因子：
11.1
作者：
PAUL, SR;BENNETT, F;YANG, YC
通讯作者：
YANG, YC
促肾上腺皮质激素增加大鼠肾上腺肾小球带细胞中白细胞介素 6 的释放。
影响因子：
4.8
作者：
Judd,AM;MacLeod,RM
通讯作者：
MacLeod,RM