Relaxin protects against renal ischemia-reperfusion injury
Relaxin protects against renal ischemia-reperfusion injury
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DOI：
10.1152/ajprenal.00654.2012
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发表时间：
2013-10-01
影响因子：
4.2
通讯作者：
Ikegaya, Naoki
中科院分区：
文献类型：
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作者：
Yoshida, Takuya;Kumagai, Hiromichi;Ikegaya, Naoki
杨志华，李志华.松弛素对肾缺血再灌注损伤的保护作用。美国肾脏生理学杂志305：F1169-F1176，2013年。首次发表于2013年8月14日; doi：10.1152/ajprenal.00654.2012.-松弛素是一种妊娠激素，具有抗凋亡和抗炎的特性。本研究的目的是确定松弛素对缺血再灌注（IR）诱导的急性肾损伤的影响。雄性大鼠进行单侧肾切除术和对侧肾IR（肾蒂夹闭45分钟）。将大鼠分为3组：1）假手术组，2）IR组，3）IR-RLX组（缺血前给予松弛素治疗）。在该组中，松弛素500 ng/h通过皮下渗透微泵输入24 h开始肾缺血前2 h。再灌注后24 h，测定肾功能，取肾进行分析。三组血压无明显差异。IR增加血浆肌酐和尿素氮水平，松弛素提供保护，防止这两个参数的增加。与IR组相比，松弛素显著降低血浆TNF-α水平和肾脏TNF受体1 mRNA表达。组织学病变的半定量评估显示，与IR-RLX大鼠相比，IR大鼠有明显的结构损伤。与IR组相比，RLX显著减少凋亡细胞计数。在IR肾脏中观察到的半胱天冬酶-3的过度表达在IR-RLX组中减少。结果表明松弛素通过减少细胞凋亡和炎症对IR诱导的肾损伤提供保护。
Yoshida T, Kumagai H, Kohsaka T, Ikegaya N. Relaxin protects against renal ischemia-reperfusion injury. Am J Physiol Renal Physiol 305: F1169-F1176, 2013. First published August 14, 2013; doi:10.1152/ajprenal.00654.2012.-Relaxin, a pregnancy hormone, has antiapoptotic and anti-inflammatory properties. The aim of this study was to determine the effects of relaxin on ischemia-reperfusion (IR)-induced acute kidney injury. Male rats underwent unilateral nephrectomy and contralateral renal IR (45 min of renal pedicle clamping). Rats were divided into three groups: 1) sham group, 2) IR group, and 3) IR-RLX group (rats treated with relaxin before ischemia). In this group, relaxin was infused at 500 ng/h via subcutaneous osmotic minipump for 24 h beginning 2 h before renal ischemia. At 24 h after reperfusion, renal function was assessed and kidneys were removed for analysis. There was no significant difference in blood pressure among the three groups. IR increased plasma levels of creatinine and urea nitrogen, and relaxin provided protection against the increases in these two parameters. Relaxin significantly decreased plasma TNF-alpha levels and renal TNF receptor 1 mRNA expression, compared with the IR group. Semiquantitative assessment of the histological lesions showed marked structural damage in IR rats compared with the IR-RLX rats. RLX significantly reduced apoptotic cell counts compared with the IR group. Overexpression of caspase-3 observed in the IR kidneys was reduced in the IR-RLX group. The results demonstrated that relaxin provided protection against IR-induced renal injury by reducing apoptosis and inflammation.