Production of Oligosaccharide from Alginate Using Pseudoalteromonas agarovorans
Production of Oligosaccharide from Alginate Using Pseudoalteromonas agarovorans
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DOI：
10.1007/s12010-008-8514-7
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发表时间：
2009-11-01
影响因子：
3
通讯作者：
Cho, Hoon
中科院分区：
文献类型：
--
作者：
Choi, DuBok;Piao, Yu Lan;Cho, Hoon
从韩国南海岸附近的海水中分离出一种海洋细菌，用于从藻酸盐中进行有效糖化。根据16S rDNA序列，分离菌株鉴定为假交替单胞菌琼脂糖菌。已在烧瓶培养物中研究了影响使用 P. agarovorans CHO-12 进行藻酸盐糖化的各种环境因素。在30 rpm和29℃下获得最佳糖浓度。在各种NaCl浓度中，当NaCl浓度从10g/l增加到30g/l时，细胞浓度急剧增加，而在40g/l以上则没有增加。当使用 30 g/l 的 NaCl 时，获得最大糖浓度为 13.8。酵母浸膏和玉米浆是高效糖化的最佳氮源。特别地，使用1.0g/l酵母提取物和1.5g/l玉米浆的混合物培养3天后获得14.9g/l的糖浓度。使用P. agarovorans CHO-12和嗜麦芽寡养单胞菌在50升反应器中进行3天的放大。当使用S. maltophilia时，细胞浓度比P. agarovorans CHO-12高约两倍。另一方面，当使用P. agarovorans CHO-12时，培养2天后获得最大糖化率，为7.5g/l/天，比嗜麦芽糖链球菌高约10倍。
A marine bacterium was isolated from seawater near the Korean south coast for efficient saccharification from alginate. Based on 16S rDNA sequence, the isolated strain was identified as Pseudoalteromonas agarovorans. Various environmental factors affecting saccharification of alginate using P. agarovorans CHO-12 have been investigated in flask cultures. The optimum concentration of sugar was obtained at 30 rpm and 29 degrees C. Among various NaCl concentrations, when NaCl concentration was increased from 10 to 30 g/l, the cell concentration sharply increased, while there is no increase at above 40 g/l. The maximum sugar concentration was obtained at 13.8 when 30 g/l of NaCl was used. Yeast extract and corn steep liquor were the best nitrogen source for efficient saccharification. Especially, the sugar concentration of 14.9 g/l was obtained after 3 days of culture using a mixture of 1.0 g/l of yeast extract and 1.5 g/l of corn steep liquor. Scale up was carried out at 50 l of reactor for 3 days using P. agarovorans CHO-12 and Stenotrophomonas maltophilia sp. When S. maltophilia was used, cell concentration was about twofold higher than that of P. agarovorans CHO-12. On the other hand, when P. agarovorans CHO-12 was used, the maximum saccharification rate was obtained, 7.5 g/l/day after 2 days of culture, which was about tenfold higher than that of S. maltophilia.