Probing Nanoparticle/Membrane Interactions by Combining Amphiphilic Diblock Copolymer Assembly and Plasmonics
Probing Nanoparticle/Membrane Interactions by Combining Amphiphilic Diblock Copolymer Assembly and Plasmonics
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DOI：
10.1021/acs.jpcb.9b10469
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
A. Koch;S. Morsbach;T. Bereau;G. Lévêque;H. Butt;M. Deserno;K. Landfester;G. Fytas
中科院分区：
文献类型：
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作者：
A. Koch;S. Morsbach;T. Bereau;G. Lévêque;H. Butt;M. Deserno;K. Landfester;G. Fytas
了解纳米颗粒（NPs）和细胞边界之间的相互作用对于其毒性和治疗应用至关重要。除了表面功能化的纳米颗粒的特异性受体介导的内吞作用之外，被动内化还受到相对非特异性参数的促进，例如粒径和电荷。基于理论处理，细胞膜的粘附和弯曲可以诱导NP包裹。实验上，需要强大的工具来选择性地探测可能的膜NP图案在非常稀的条件下，避免染料标记。在这项工作中，我们采用表面共振增强的动态光散射，表面等离子体共振，电子显微镜和模拟等离子体纳米金颗粒和聚合物之间的相互作用。我们区分三种不同的相互作用方案在纳摩尔浓度通过调整金纳米粒子的表面电荷和合理化这些事件通过平衡囊泡弯曲和静电/货车德瓦尔斯金纳米粒子和囊泡粘附。澄清的物理条件下，纳米粒子被动跨膜转运可以帮助合理设计的药物，不能利用特定的细胞摄取模式，也阐明了物理特性，使纳米粒子在环境中特别有毒。
Understanding the interactions between nanoparticles (NPs) and boundaries of cells is crucial both for their toxicity and therapeutic applications. Besides specific receptor-mediated endocytosis of surface-functionalized NPs, passive internalization is prompted by relatively unspecific parameters, such as particle size and charge. Based on theoretical treatments, adhesion to and bending of the cell membrane can induce NP wrapping. Experimentally, powerful tools are needed to selectively probe possible membrane-NP motifs at very dilute conditions and avoid dye labeling. In this work, we employ surface resonance-enhanced dynamic light scattering, surface plasmon resonance, electron microscopy, and simulations for sensing interactions between plasmonic AuNPs and polymersomes. We distinguish three different interaction scenarios at nanomolar concentrations by tuning the surface charge of AuNPs and rationalize these events by balancing vesicle bending and electrostatic/van der Waals AuNP and vesicle adhesion. The clarification of the physical conditions under which nanoparticles passively translocate across membranes can aid in the rational design of drugs that cannot exploit specific modes of cellular uptake and also elucidates physical properties that render nanoparticles in the environment particularly toxic.