Effects of different ultrasound frequencies on the structure, rheological and functional properties of myosin: Significance of quorum sensing
Effects of different ultrasound frequencies on the structure, rheological and functional properties of myosin: Significance of quorum sensing
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DOI：
10.1016/j.ultsonch.2020.105268
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发表时间：
2020-12-01
影响因子：
8.4
通讯作者：
Xu, Xinglian
中科院分区：
文献类型：
--
作者：
Chen, Jiahui;Zhang, Xing;Xu, Xinglian
首次比较了单频脉冲超声(SFPU，G1-G2)和双频脉冲超声(DFPU，G3)对肌原纤维蛋白(MP)中肌球蛋白的结构和流变性的影响。结果表明，SFPU和DFPU通过空化作用于多个肌球蛋白而诱导“应激反应”。此外，肌球蛋白之间可能存在一定的群体感应，诱导出更稳定的β-反平行结构来抵抗空化力的负面影响。颗粒大小和同步荧光分析结果表明，MPS中肌球蛋白的结构在应力作用下发生了变化。PH的升高也有助于超声过程(G5-G7)。值得注意的是，DFPU诱导了更强的群体感应并形成了更稳定的结构。此外，还考察了表没食子儿茶素没食子酸酯(EGCG)和黄芩素(BN)对DFPU处理和未处理MPS乳液和凝胶性能的影响。结果表明，超声波对乳状液的稳定性有一定的增强作用。此外，凝胶的质构和可表达水分含量(Emoc)也在处理后有所改善。
Structure and rheological properties of myosin in myofibrillar protein (MP) after single frequency pulsed ultrasound (SFPU, G1-G2) and dual frequency pulsed ultrasound (DFPU, G3) were compared for the first time. Results showed SFPU and DFPU induced "stress response" through the action of cavitation on multiple myosin. In addition, there may be a certain quorum sensing among myosin, inducing a more stable beta-antiparallel structure to resist negative effects of cavitation force. Results of particle size and synchronous fluorescence indicated that structure of myosin in MPs changed through stress. The increase in pH also assisted in the ultrasound process (G5-G7). Notably, DFPU induced stronger quorum sensing and formed a more stable structure. More so, effects of (-)-epigallocatechin-3-gallate (EGCG) and baicalein (BN) on the emulsion and gel properties of DFPU treated and non-treated MPs were also investigated. Results showed that ultrasound increased the stability of emulsion. Additionally, the texture and expressible moisture content (EMOC) of the gel were also improved after treatment.