DNA rendering of polyhedral meshes at the nanoscale
DNA rendering of polyhedral meshes at the nanoscale
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DOI：
10.1038/nature14586
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发表时间：
2015-07-23
期刊：
影响因子：
64.8
通讯作者：
Hogberg, Bjorn
中科院分区：
文献类型：
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作者：
Benson, Erik;Mohammed, Abdulmelik;Hogberg, Bjorn
有人建议（1）超过三十年前，沃森-克里克碱基配对可能用于合理设计的纳米尺度结构的核酸。从那时起，特别是自从折纸技术的引入（2），DNA纳米技术已经使越来越复杂的结构（3-18）。但是，尽管用于创建DNA折纸多边形网格和设计软件的一般方法是可用的（14，16，17，19 -21），但是仍然存在由DNA几何形状和有义/反义配对引起的重要约束，需要在设计过程中进行一些手动调整。在这里，我们提出了一种通用的方法，折叠任意多边形的数字网格的DNA，很容易产生的结构，将是非常难以实现使用以前的方法。设计过程是高度自动化的，使用基于图论的路由算法和通过目标结构追踪支架链的松弛模拟。此外，与从紧密堆积的螺旋构建的传统折纸设计不同，我们的结构具有更开放的构象，每个边缘具有一个螺旋，因此在生物测定中通常使用的离子条件下稳定。
It was suggested(1) more than thirty years ago that Watson-Crick base pairing might be used for the rational design of nanometre-scale structures from nucleic acids. Since then, and especially since the introduction of the origami technique(2), DNA nanotechnology has enabled increasingly more complex structures(3-18). But although general approaches for creating DNA origami polygonal meshes and design software are available(14,16,17,19-21), there are still important constraints arising from DNA geometry and sense/antisense pairing, necessitating some manual adjustment during the design process. Here we present a general method of folding arbitrary polygonal digital meshes in DNA that readily produces structures that would be very difficult to realize using previous approaches. The design process is highly automated, using a routeing algorithm based on graph theory and a relaxation simulation that traces scaffold strands through the target structures. Moreover, unlike conventional origami designs built from close-packed helices, our structures have a more open conformation with one helix per edge and are therefore stable under the ionic conditions usually used in biological assays.