Intra-amygdala infusion of the N-methyl-D-aspartate receptor antagonist AP5 blocks acquisition but not expression of fear-potentiated startle to an auditory conditioned stimulus.
Intra-amygdala infusion of the N-methyl-D-aspartate receptor antagonist AP5 blocks acquisition but not expression of fear-potentiated startle to an auditory conditioned stimulus.
复制标题
杏仁核内注射 N-甲基-D-天冬氨酸受体拮抗剂 AP5 可阻止对听觉条件刺激的恐惧增强惊吓的获得，但不会阻止其表达。
DOI：
10.1037//0735-7044.106.3.569
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发表时间：
1992
影响因子：
1.9
通讯作者：
Davis,M
中科院分区：
文献类型：
--
作者：
Campeau,S;Miserendino,MJ;Davis,M
恐惧增强惊吓范式，其中惊吓反射的幅度在先前与足电击配对的刺激的存在下增强，用于测量杏仁核内输注竞争性N-甲基-D-天冬氨酸（NMDA）受体拮抗剂DL-2-氨基-5-膦酰基戊酸（AP 5）后的厌恶性条件反射。在连续2天的5次噪声-足电击配对之前立即输注2.5 µg/侧AP 5，剂量依赖性地阻断听觉恐惧增强惊吓的获得或巩固，与我们实验室先前用视觉刺激获得的结果一致。躯体感觉或听觉传递缺陷似乎不诱导杏仁核内AP 5，因为大鼠反应正常，足电击，并表现出可靠的增强惊吓表达后，预测试AP 5输液的剂量，阻止收购。与早期的报告一起，这些数据表明，位于杏仁核内或附近的NMDA依赖性过程可能是获得不同感觉方式的条件性恐惧所必需的。
The fear-potentiated startle paradigm, in which the amplitude of the startle reflex is enhanced in the presence of a stimulus previously paired with footshock, was used to measure aversive conditioning after intra-amygdala infusion of the competitive N-methyl-D-aspartate (NMDA) receptor antagonist DL-2-amino-5-phosphonopentanoic acid (AP5). Infusion of 2.5 µg/side AP5 immediately before five noise–footshock pairings on each of 2 consecutive days dose-dependently blocked acquisition or consolidation of auditory fear-potentiated startle, consistent with previous results from our laboratory obtained with a visual stimulus. Somatosensory or auditory transmission deficits do not appear to be induced by intra-amygdala AP5 because rats reacted normally to footshocks and showed reliable potentiated startle expression after pretesting AP5 infusion at a dose that blocked acquisition. Together with earlier reports, these data suggest that an NMDA-dependent process localized in or near the amygdala may be necessary for the acquisition of conditioned fear across different sensory modalities.