Massilia flava sp. nov., isolated from soil.
Massilia flava sp. nov., isolated from soil.
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DOI：
10.1099/ijs.0.031344-0
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发表时间：
2012-03
影响因子：
2.8
通讯作者：
Jiewei Wang;Jianli Zhang;H. Pang;Yabo Zhang;Yuyi Li;Jinping Fan
中科院分区：
文献类型：
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作者：
Jiewei Wang;Jianli Zhang;H. Pang;Yabo Zhang;Yuyi Li;Jinping Fan
从宁夏土壤中分离到一株革兰氏染色阴性的杆状细菌Y 9（T），并对其进行了分类鉴定。菌株Y 9（T）含有Q-8作为主要的泛醌。主要脂肪酸组分是特征3（C（16：1）ω 7 c和/或异-C（15：0）2-OH）和C（16：0）的总和。主要极性脂质为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。菌株Y 9（T）基因组DNA的G+C含量为68.7mol%。基于16 S rRNA基因序列的系统发育树显示，该菌株属于Massilia属所涵盖的进化辐射。Y 9（T）菌株与已知的Massilia种的模式菌株16 S rRNA基因序列相似性为95.2 ~ 98.2%，其中与白黄Massilia 45（T）和黄Massilia lutea 101（T）的相似性最高，分别为98.2%和98.0%。然而，菌株Y 9（T）和M. albidiflavaKCTC 12343（T）和M. lutea KCTC 12345（T）分别为37%和26%。菌株Y 9（T）与其最近的系统发育亲缘关系在表型、化学分类学和系统发育学特征上明显不同。因此，菌株Y 9（T）被认为代表了一个新的种的Massilia属，并建议将其命名为Massilia flava sp. nov.。模式菌株为Y 9（T）（=CGMCC 1.10685（T）=KCTC 23585（T））。
A Gram-stain-negative, rod-shaped bacterium, designated strain Y9(T), was isolated from a soil sample collected in Ningxia Province in China and was characterized to determine its taxonomic position. Strain Y9(T) contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) and C(16:0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain Y9(T) was 68.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Massilia. Levels of 16S rRNA gene sequence similarity between strain Y9(T) and the type strains of recognized Massilia species ranged from 95.2 to 98.2%, the highest values being with Massilia albidiflava 45(T) (98.2%) and Massilia lutea 101(T) (98.0%). However, levels of DNA-DNA relatedness between strain Y9(T) and M. albidiflava KCTC 12343(T) and M. lutea KCTC 12345(T) were 37 and 26%, respectively. Strain Y9(T) was clearly differentiated from its nearest phylogenetic relatives in the genus Massilia based on phenotypic, chemotaxonomic and phylogenetic properties. Therefore, strain Y9(T) is considered to represent a novel species of the genus Massilia, for which the name Massilia flava sp. nov. is proposed. The type strain is Y9(T) (=CGMCC 1.10685(T) =KCTC 23585(T)).