Early maternal separation increases NGF expression in the developing rat hippocampus
Early maternal separation increases NGF expression in the developing rat hippocampus
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DOI：
10.1016/s0091-3057(97)00512-1
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发表时间：
1998-04-01
影响因子：
3.6
通讯作者：
Aloe, L
中科院分区：
文献类型：
--
作者：
Cirulli, F;Micera, A;Aloe, L
神经生长因子（Nerve Growth Factor，NGF）是一种神经营养因子，参与中枢胆碱能神经元的生长和分化。在这项研究中，母体分离的范例被用来测试是否神经生长因子的水平可能会受到影响，在个体发育的早期大鼠幼崽的简单操作。采用原位杂交技术检测了3日龄大鼠仔鼠在母体分离45 min后神经生长因子mRNA的表达。早期母亲分离导致新生大鼠海马齿状回和门区NGFmRNA表达增加。分离程序后第二天在整个海马中测量的NGF蛋白水平（通过灵敏的ELISA测定）在分离与未分离的幼崽中没有差异。这些数据表明，在发育早期进行的简单操作可以影响海马神经生长因子的表达。(C)1998年爱思唯尔科学公司
Nerve Growth Factor (NGF) is a neurotrophin involved in growth and differentiation of central cholinergic neurons. In this study a maternal separation paradigm was used to test whether levels of NGF might be affected by brief manipulations of rat pups early during ontogeny. The expression of NGF mRNA was examined in 3-day-old rat pups following 45 min maternal separation using in situ hybridization. Early maternal separation in neonatal rats resulted in increased expression of NGF mRNA in the dentate gyrus and the hilus of the hippocampus. NGF protein levels measured (by means of a sensitive ELISA assay) in the whole hippocampus the day following the separation procedure did not differ in separated vs. nonseparated pups. These data indicate that brief manipulations performed early during development can affect hippocampal NGF expression. (C) 1998 Elsevier Science Inc.