On-Chip Gel-Valve Using Photoprocessable Thermoresponsive Gel
On-Chip Gel-Valve Using Photoprocessable Thermoresponsive Gel
复制标题
使用可光处理热响应凝胶的片上凝胶阀
DOI：
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
F. Arai
中科院分区：
文献类型：
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作者：
Keitaro Ito;S. Sakuma;Y. Yokoyama;F. Arai
微流控芯片是生物化学实验的有力工具。通过使用芯片上的微型阀可以实现高速和精确的流量控制。已经报道了可以集成到微流体芯片中的几种类型的微阀。其中，凝胶微阀由于其柔软的结构而具有一定的优势，这将有助于防止通过阀的细胞的机械损伤。在这里，我们使用Bioresist，一种可光处理的热敏凝胶，作为微阀的关键组成部分。由于Bioresist是可图案化的，我们可以使用光刻法从热敏凝胶中创建任何任意的2D形状。此外，Bioresist具有在30°C左右发生相变的独特特征，并随着温度的变化反复膨胀和收缩。通过将图案化的温敏凝胶与微加热器相结合，我们研制了一种凝胶致动器，并设计了一种凝胶阀。凝胶阀具有简单的驱动机构的优点：高泄漏压力，高速驱动和低功耗。该瓣膜具有生物相容性，并且通过使用传统的光刻法容易地集成到芯片中。利用该阀实现了芯片上的流量控制，并将其应用于芯片上的细胞分选。
Microfluidic chips are powerful tools for biochemical experiments. High speed and precise flow control can be achieved by using microvalves on a chip. Several types of microvalves that can be integrated into a microfluidic chip have been reported. Among them, gel microvalves have certain advantages over other valves because of their soft structure, which will contribute to prevent mechanical damage the cells passing though the valve. Here we use Bioresist, a photoprocessable thermoresponsive gel, as a key component of the microvalve. Since Bioresist is photopatternable, we can create any arbitrary 2D shape from the thermoresponsive gel using photolithography. Moreover, Bioresist has the unique feature of a phase transition around 30°C, and swells and shrinks repeatedly with temperature change. By integrating the patterned thermoresponsive gel with a microheater, we developed a gel actuator and designed a gel-valve. The gel-valve has the advantages of a simple actuation mechanism: high leakage pressure, high speed actuation and low power consumption. The valve is biocompatible and easily integrated into a chip by using conventional photolithography. Using this valve, we achieved on-chip flow control, and applied it to cell sorting on a chip.