Serological evidence of Brucellosis in selected gaushalas of Haryana
Serological evidence of Brucellosis in selected gaushalas of Haryana
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哈里亚纳邦选定高沙拉斯中布鲁氏菌病的血清学证据
DOI：
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发表时间：
2020
影响因子：
0.4
通讯作者：
R. Chhabra
中科院分区：
文献类型：
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作者：
A. Saidu;N. Mahajan;Mahavir Singh;D. Mittal;Bangar Yogesh;R. Chhabra
布鲁氏菌病是一种被忽视的人畜共患疾病，在发展中国家对人类和动物种群造成重大的经济和公共卫生后果。本研究的目的是确定在哈里亚纳邦希萨尔和金德区的两个gaushala饲养的牛中布鲁氏菌病的血清学证据。血清学试验：采用虎红平板试验（RBPT）、血清凝集试验（SAT）和酶联免疫吸附试验（ELISA）对动物进行布鲁氏菌病筛查。RBPT、SAT和ELISA的总阳性率分别为23.46%、20.67%和28.49%。逻辑回归模式得出结论，年龄> 6岁，随后为3-6岁的奶牛比年龄<3岁的奶牛患布鲁氏菌病的可能性更高。经Kappa检验，RBPT与ELISA、SAT与ELISA、RBPT与SAT的符合率分别为0.87（95% CI：0.857-0.882）、0.70（95% CI：0.684-0.718）和0.82（95% CI：0.809-0.834）。本研究得出结论，在高沙拉，动物作为封闭种群饲养，年龄较大的挤奶动物中布鲁氏菌病的风险更高，这通过食用未经巴氏消毒的牛奶构成潜在的公共卫生风险。
Brucellosis is a neglected zoonotic disease with significant economic and public health consequences to human and animal population in developing countries. The objective of the present study was to determine the serological evidences of brucellosis in cattle reared in two gaushalas of Hisar and Jind districts, Haryana. The serological tests: Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme Linked Immunosorbent Assay (ELISA) were employed for screening the animals for brucellosis. The overall seropositivity by RBPT, SAT and ELISA was 23.46%, 20.67% and 28.49% respectively. The logistic regression modalities concluded higher likelihood of brucellosis with age > 6 years followed by 3–6 year than cows with <3 years. The agreement between tests (RBPT and ELISA, SAT and ELISA and RBPT and SAT) was found to be 0.87 (95% CI: 0.857–0.882), 0.70 (95% CI: 0.684–0.718) and 0.82 (95% CI: 0.809–0.834) respectively by kappa statistic. This study concluded high infection rate in gaushala where animals were kept as closed population with more risks of brucellosis among older milching animals which poses potential public health risk through consumption of unpasteurized milk.