Molecular epidemiology of human polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa
Molecular epidemiology of human polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa
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DOI：
10.1590/s0074-02761998000500010
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发表时间：
1998-09-01
影响因子：
2.8
通讯作者：
Stoner, GL
中科院分区：
文献类型：
--
作者：
Chima, SC;Ryschkewitsch, CF;Stoner, GL
JC多瘤病毒（Polyomavirus JC，JCV）是一种广泛存在于人类的病毒，可引起中枢神经系统的慢性脱髓鞘疾病，即进行性多灶性白质脑病，在艾滋病（AIDS）中常见。JCV在全球30-70%的成人尿液中排泄。基于JCV完整基因组或其片段的序列分析，JCV可以被分类为地理来源的基因型。1型和2型分别来自欧洲和亚洲，而3型和6型则来自非洲。4型，可能是欧洲和非洲基因型（1和3）的重组体，在美国很常见。为了描述非洲土著人群中的JCV基因型，从中非共和国的Biaka俾格米人和班图人中随机收集尿样。男43例，女25例，年龄4-55岁，平均26岁。PCR扩增尿液中的JCV后，产物直接进行循环测序。23名俾格米成人中有5名（22%）和20名班图成人中有4名（20%）为JC病毒尿阳性。DNA序列分析显示JCV 6型。JCV的3型和6型菌株是中非的优势菌株。在Biaka俾格米人中存在多种JCV亚型可能是俾格米人在赤道森林中巡回运动期间与其非洲部落邻居广泛互动的结果。
Polyomavirus JC (JCV) is ubiquitous in humans and causes a chronic demyelinating disease of the central nervous system, progressive multifocal leukoencephalopathy which is common in AIDS. JCV is excreted in urine of 30-70% of adults worldwide. Based on sequence analysis of JCV complete genomes or fragments thereof, JCV can be classified into geographically derived genotypes. Types 1 and 2 are of European and Asian origin respectively while Types 3 and 6 are African in origin. Type 4, a possible recombinant of European and African genotypes ( 1 and 3) is common in the USA. To delineate the JCV genotypes in an aboriginal African population, random urine samples were collected from the Biaka Pygmies and Bantu from the Central African Republic. There were 43 males and 25 females aged 4-55 years, with an average age of 26 years. After PCR amplification of JCV in urine, products were directly cycle sequenced. Five of 23 Pygmy adults (22%) and four of 20 Bantu adults (20%) were positive for JC viruria. DNA sequence analysis revealed JCV Type 6. Type 3 and 6 strains of JCV are the predominant strains in central Africa. The presence of multiple subtypes of JCV in Biaka Pygmies may be a result of extensive interactions of Pygmies with their African tribal neighbors during their itinerant movements in the equatorial forest.