Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method
Development of a non-viral multifunctional envelope-type nano device by a novel lipid film hydration method
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DOI：
10.1016/j.jconrel.2004.04.024
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发表时间：
2004-08-11
影响因子：
10.8
通讯作者：
Harashima, H
中科院分区：
文献类型：
--
作者：
Kogure, K;Moriguchi, R;Harashima, H
描述了使用新型脂质膜水合方法开发用于非病毒基因传递系统的多功能包膜型纳米装置（MEND）。该包装方法涉及三个步骤：（i）用聚阳离子缩合DNA，（ii）脂质膜水合以静电结合缩合DNA，以及（iii）超声处理以将缩合DNA与脂质包装。对于 DNA 浓缩，DNA 与聚 L-赖氨酸 (PLL) 的最佳比例通过滴定 DNA 和 PLL 来确定。通过脂质膜水合和超声处理，浓缩的 DNA/PLL 复合物 (DPC) 被脂质双层包被，并通过蔗糖密度梯度离心证实。用脂质包装 DPC 的转染活性比 DPC 提高了 10 倍。 MEND 的包膜上含有八精氨酸，作为膜穿透装置以增强细胞摄取，其转染活性比 DPC 高 1000 倍。本研究中开发的 MEND 代表了一种有前途的非病毒基因传递系统。 (C) 2004 Elsevier B.V. 保留所有权利。
The development of a multifunctional envelope-type nano device (MEND) for use in a non-viral gene delivery system using a novel lipid film hydration method is described. This packaging method involves three steps: (i) DNA condensation with a polycation, (ii) lipid film hydration for the electrostatic binding of the condensed DNA, and (iii) sonication to package the condensed DNA with lipids. For DNA condensation, the optimum ratio of DNA to poly-L-lysine (PLL) was determined by titrating DNA and PLL. The condensed DNA/PLL complex (DPC) was coated with a lipid bilayer by lipid film hydration followed by sonication, which was confirmed by sucrose density gradient centrifugation. Packaging of DPC with lipids increased the transfection activity 10-fold over that of DPC. MEND, having octaarginine on the envelope as a device for membrane penetration to enhance cellular uptake, showed a 1000-fold higher transfection activity than DPC. The MEND developed in this study represents a promising non-viral gene delivery system. (C) 2004 Elsevier B.V. All rights reserved.