Comparison of the protective effects of truncated bFGF and native bFGF against murine lung carcinoma.
Comparison of the protective effects of truncated bFGF and native bFGF against murine lung carcinoma.
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DOI：
10.3892/ijmm.2011.676
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发表时间：
2011-07
影响因子：
5.4
通讯作者：
Zi-chao Luo;Xin-Yun Peng;Hua-Shan Shi;C. Gong;Z. Qian;Li Yang
中科院分区：
文献类型：
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作者：
Zi-chao Luo;Xin-Yun Peng;Hua-Shan Shi;C. Gong;Z. Qian;Li Yang
碱性成纤维细胞生长因子（bFGF）是一种血管生成因子，通过与酪氨酸激酶受体和肝素硫酸化蛋白聚糖受体相互作用而发挥促血管生成作用。本研究设计了一种N、C端截短的碱性成纤维细胞生长因子（tbFGF），并将其与野生型bFGF进行比较，用于以PCEC水凝胶为佐剂的小鼠肺癌免疫治疗。在体外，tbFGF没有刺激NIH-3 T3成纤维细胞增殖。在体内，免疫后，tbFGF和bFGF都能够诱导强有力的bFGF特异性免疫应答。保护性抗肿瘤研究显示，在tbFGF或bFGF组中，通过免疫组织化学染色和藻酸盐包封的肿瘤细胞测定检测到肿瘤生长的显著抑制和肿瘤血管化的减少。这些数据表明，tbFGF可用于肿瘤的免疫治疗，而没有与bFGF相关的风险，其在正常组织中诱导新血管形成。
Basic fibroblast growth factor (bFGF), an angiogenic factor, exhibits pro-angiogenic abilities by interacting with tyrosine kinase receptors and heparin-sulfated proteoglycan receptors. Here, we designed an N-, C-terminally truncated basic fibroblast growth factor (tbFGF) for immuno-therapy of murine lung carcinoma with PCEC hydrogel as adjuvant, comparing it with the wild-type bFGF. In vitro, tbFGF did not stimulate NIH-3T3 fibroblast proliferation. In vivo, after immunization, both tbFGF and bFGF were able to induce a robust bFGF-specific immune response. The protective anti-tumor investigation showed a significant inhibition of tumor growth and reduction of tumor vascularization detected by immunohistochemical staining and the alginate-encapsulated tumor cell assay in the tbFGF or the bFGF group. These data suggested that tbFGF can be used in the immunotherapy of tumors, without the risks associated with bFGF, which induces neovascularization in normal tissues.