The dynamics of clot formation in fresh-frozen plasma
The dynamics of clot formation in fresh-frozen plasma
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DOI：
10.1111/j.1423-0410.2008.01037.x
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发表时间：
2008-01-01
期刊：
影响因子：
2.7
通讯作者：
Mackie, I. J.
中科院分区：
文献类型：
--
作者：
Lawrie, A. S.;Cardigan, R. A.;Mackie, I. J.
背景因子 VIII (FVIII) 水平用作新鲜冰冻血浆 (FFP) 的质量标志；然而，其他凝血因子并未常规测量。 方法 我们使用血栓弹力图 (TEG (R)) 和凝血酶生成测试 (TGT) 评估其他止血参数和凝血动力学。 FFP 在捐献当天（第 0 天）或在 4°C 下过夜（第 1 天）后制备。结果 第 1 天 FFP 中的因子 VIII 比第 0 天低 18%。第 1 天 FFP 中的 TEG (R) 参数与凝血性增加一致，与凝血因子水平改变无关，但与第 1 天样本中观察到的微粒水平增加一致。 TGT 研究显示滞后时间、达到峰值的时间增加，凝血酶峰值产生减少，但第 1 天内源性凝血酶电位 (ETP) 没有变化。FVIII 水平与 ETP 和凝血酶峰值之间存在微弱关联 (ETP r(s) >= 0.22，P = 0.48，P
Background Factor VIII (FVIII) levels are used as a quality marker of fresh-frozen plasma (FFP); however, other clotting factors are not routinely measured.Methods We assessed additional haemostatic parameters and the dynamics of coagulation using Thrombelastography (TEG (R)) and a thrombin generation test (TGT). FFP was prepared on the day of donation (Day 0) or after overnight hold at 4 degrees C (Day 1).Results Factor VIII in Day 1 FFP was 18% lower than in Day 0. TEG (R) parameters in Day 1 FFP were consistent with increased coagulability and did not correlate with altered levels of clotting factors, but were consistent with the increased levels of microparticles seen in the Day 1 samples. TGT studies exhibited increased lag time, time to peak and reduced peak thrombin generation, but no change in endogenous thrombin potential (ETP) on Day 1. There was a weak association between FVIII level and both ETP and peak thrombin (ETP r(s) >= 0.22, P = 0.48, P