Experimental induction of neurofibromatosis in bicolor damselfish
Experimental induction of neurofibromatosis in bicolor damselfish
复制标题
双色雀鲷神经纤维瘤病的实验诱导
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
M. Schmale
中科院分区：
文献类型：
--
作者：
M. Schmale
Damselfish ileurofibromatosis (DNF) is a neoplastic disease affecting the peripheral nervous system and chromatophores of bicolor damselfish Pornacentruspartitus on Florida (USA) reefs. This report presents the results of transmission experiments using intramuscular and intraperitoneal injection of a variety of tumor preparations to induce DNF. Neurofibromas developed within 1 yr of injection in 76% of fish injected with homogenates of spontaneously occurring tumors with earliest tumor development occurring at 5 to 6 wk post-injection. Cell-free transmission using 0.2 pm filtrates of DNF tumor homogenates was observed, demonstrating that the agent responsible for DNF is subcellular. Four out of five tumor cell lines established from neurofibromas were found to be tumorigenic when injected, while cells cultured from normal fish had no effect, indicating that the etiologic agent of DNF was present and infectious in tumor cell lines but not in cell lines or primary cultures derived from healthy fish. Serial passages of tumors were conducted through 19 passage generations in vivo. A comparison of the cumulative time to tumor distributions showed that most experiments beyond the third serial passage yielded significantly faster tumor development than was obtained by passaging from spontaneous tumors. Induced tumors exhibited more localized growth patterns and were typically more rapidly g]-owing and more rapidly lethal than spontaneous tumors. In contrast to the differences in growth patterns observed, the histological phenotype of the spontaneous, wild-type DNF lesions was indistinguishable from that of the induced tumors obtained from serial passages as well as in experiments involv~ng cell-free filtrates and cultured tumor cells. These observations indicate that the process of neoplastic transformation is relatively stable in this system, \nth little or no phenotypic drift caused by these manipulations. Naturally occurring, transmissible cancers that arise from cells of the central or peripheral nervous system or from chromatophores have not been previously identified. Thus, DNF is a unique disease and may provide an important model for investigating thls rather unique form of viral oncogenesis.
双色雀鲷神经纤维瘤病样疾病的传染性。
影响因子：
11.2
作者：
Schmale,MC;Hensley,GT
通讯作者：
Hensley,GT
感染劳斯肉瘤病毒的鸡中，炎症是导致伤口诱发肿瘤的原因。
影响因子：
11.2
作者：
Martins-Green,M;Boudreau,N;Bissell,MJ
通讯作者：
Bissell,MJ
患有神经纤维瘤病的雀鲷对肿瘤靶点表现出细胞毒性。
影响因子：
2.9
作者：
McKinney,EC;Schmale,MC
通讯作者：
Schmale,MC