Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.
Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.
复制标题
通过二维场反转凝胶电泳技术对支原体移动基因组进行快速物理绘图。
DOI：
10.1093/nar/16.24.11461
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发表时间：
1988
影响因子：
14.9
通讯作者：
Wilfried Bautsch
中科院分区：
文献类型：
--
作者：
Wilfried Bautsch
构建了780 kbp的移动的支原体（ATCC 43663）基因组的宏观限制性酶切图谱。在二维脉冲场电泳凝胶上鉴定连接片段。无论是完整的双限制性酶或部分和完整的单限制性酶分别在第一和第二维分离。在该图谱上可定位出4种酶的19个限制性位点。这些快速方法不需要DNA探针，适用于所有基因组的长距离限制性作图，在二维凝胶上产生可分辨的模式。
A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzymes could be assigned to the map. These rapid methods do not require DNA probes and are applicable to the long-range restriction mapping of all genomes that yield resolvable patterns on two-dimensional gels.