Natural Clearance of Chlamydia trachomatis Infection Is Associated With Distinct Differences in Cervicovaginal Metabolites.
Natural Clearance of Chlamydia trachomatis Infection Is Associated With Distinct Differences in Cervicovaginal Metabolites.
复制标题
沙眼衣原体感染的自然清除与宫颈阴道代谢物的明显差异相关。
DOI：
10.1093/infdis/jiad155
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Geisler,WilliamM
中科院分区：
文献类型：
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作者：
Jordan,StephenJ;Wilson,Landon;Ren,Jie;Gupta,Kanupriya;Barnes,Stephen;Geisler,WilliamM
女性沙眼衣原体的自然清除发生在筛查和治疗之间。在体外，干扰素-γ (IFN-γ)介导的色氨酸消耗导致沙眼清除，但这种机制是否发生在体内尚不清楚。我们之前发现，自然清除dc的女性。与持续感染的女性相比，沙眼患者的宫颈阴道色氨酸和IFN-γ水平较低，这表明IFN-γ不依赖通路可能促进tec。trachomatisclearance。方法对34例自然清除（n = 17）或未清除（n = 17）的妇女进行阴道灌洗。沙眼采用非靶向高效液相色谱质谱法鉴定与自然清除相关的代谢物和代谢途径。结果共标注375个带正电荷代谢物和149个带负电荷代谢物。与持续感染的妇女相比，C。沙眼的自然清除与低聚糖海藻糖、蔗糖、麦芽糖糖和麦芽糖糖水平的增加以及吲哚啉和各种氨基酸水平的降低有关。代谢物与缬氨酸、亮氨酸和异亮氨酸的生物合成途径有关。结论自然清除或未清除arc的妇女的宫颈阴道代谢组。trachomatisis截然不同。在清除dc的女性中。沙眼虫，各种氨基酸，特别是缬氨酸，亮氨酸和异亮氨酸的消耗，表明除色氨酸以外的氨基酸的影响。沙眼在体内存活。
BackgroundNatural clearance ofChlamydia trachomatisin women occurs in the interval between screening and treatment. In vitro, interferon-γ (IFN-γ)–mediated tryptophan depletion results inC. trachomatisclearance, but whether this mechanism occurs in vivo remains unclear. We previously found that women who naturally clearedC. trachomatishad lower cervicovaginal levels of tryptophan and IFN-γ compared to women with persisting infection, suggesting IFN-γ–independent pathways may promoteC. trachomatisclearance.MethodsCervicovaginal lavages from 34 women who did (n = 17) or did not (n = 17) naturally clearC. trachomatiswere subjected to untargeted high-performance liquid chromatography mass-spectrometry to identify metabolites and metabolic pathways associated with natural clearance.ResultsIn total, 375 positively charged metabolites and 149 negatively charged metabolites were annotated. Compared to women with persisting infection,C. trachomatisnatural clearance was associated with increased levels of oligosaccharides trehalose, sucrose, melezitose, and maltotriose, and lower levels of indoline and various amino acids. Metabolites were associated with valine, leucine, and isoleucine biosynthesis pathways.ConclusionsThe cervicovaginal metabolome in women who did or did not naturally clearC. trachomatisis distinct. In women who clearedC. trachomatis, depletion of various amino acids, especially valine, leucine, and isoleucine, suggests that amino acids other than tryptophan impactC. trachomatissurvival in vivo.