Facile Strategy for Visible Disassembly of Spherical Nucleic Acids Programmed by Catalytic DNA Circuits
Facile Strategy for Visible Disassembly of Spherical Nucleic Acids Programmed by Catalytic DNA Circuits
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由催化 DNA 电路编程的球形核酸可见分解的简便策略
DOI：
10.1021/acsami.9b02107
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发表时间：
2019
影响因子：
9.5
通讯作者：
Liang Haojun
中科院分区：
文献类型：
--
作者：
Wei Bing;Yao Dongbao;Zheng Bin;Zhou Xiang;Guo Yijun;Li Xiang;Li Chengxu;Xiao Shiyan;Liang Haojun
可编程支点介导的dna链位移反应在驱动球形核酸组装方面表现出非凡的能力。在这里，我们开发了一种简单的策略，将基于DNA链位移的DNA电路与通用的球形核酸聚集体系统集成，用于球形核酸的可见拆卸。该系统具有快速的比色反应和良好的灵敏度，可用于单核苷酸多态性的鉴别。此外，将固定球形核酸拆卸系统与两个DNA电路相结合，构建了用于多路检测的OR逻辑门。这一策略在制造便携式低成本DNA诊断试剂盒方面具有巨大的潜力，并且在其他应用领域，如复杂的DNA网络和纳米粒子超晶格的可编程相变方面，也是一种非常有前途的方法。
The programmable toehold-mediated DNA-strand-displacement reaction has demonstrated its extraordinary capability in driving the spherical nucleic acid assembly. Here, a facile strategy of integrating a DNA-strand-displacement-based DNA circuit with a universal spherical nucleic acid aggregate system was developed for the visible disassembly of spherical nucleic acids. This integrated system exhibited rapid colorimetric response and good sensitivity in the disassembly reaction and demonstrated its capability in the application of single nucleotide polymorphism discrimination. Moreover, an OR logic gate used for multiplex detection was constructed through combining the fixed spherical nucleic acid disassembly system with two DNA circuits. This strategy will have great potential in the fabrication of a portable low-cost DNA diagnostic kit, and it is also a very promising method to be used in other applications, such as complex DNA networks and programmable phase transformation of nanoparticle superlattices.