Effects of spatial confinement on migratory properties of Dictyostelium discoideum cells.
Effects of spatial confinement on migratory properties of Dictyostelium discoideum cells.
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DOI：
10.1080/19420889.2021.1872917
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发表时间：
2021-01-28
影响因子：
--
通讯作者：
Weijer CJ
中科院分区：
文献类型：
--
作者：
Belotti Y;McGloin D;Weijer CJ
各种真核细胞，如变形虫，白细胞和癌细胞的迁移环境，通常涉及空间限制。最近出现了许多研究，旨在开发更好地概括细胞微环境特征的实验平台。使用微流控技术，我们表明，越来越多的限制Dictyosteelicdiscoideum细胞进入较窄的微通道导致迁移模式和相关的肌动球蛋白细胞骨架的安排的显着变化。我们观察到细胞倾向于以恒定的速度迁移，其大小取决于通道的大小，每个细胞所采取的运动策略也是如此。观察到两种不同的迁移模式，伪足为基础的迁移和水泡为基础的迁移，水泡为基础的迁移更频繁，增加限制，并导致迁移速度较慢。除了迁移模式外，我们发现细胞速度的主要决定因素是其突起率，其前缘的F-肌动蛋白量和肌动蛋白灶的数量。我们的研究结果强调了微环境对细胞行为的影响。此外，我们开发了一种新的定量运动分析平台，用于一维细胞迁移，可以标准化和简化实验条件，并有助于研究在单细胞水平上发生的复杂和动态过程。
Migratory environments of various eukaryotic cells, such as amoeba, leukocytes and cancer cells, typically involve spatial confinement. Numerous studies have recently emerged, aimed to develop experimental platforms that better recapitulate the characteristics of the cellular microenvironment. Using microfluidic technologies, we show that increasing confinement of Dictyostelium discoideum cells into narrower micro-channels resulted in a significant change in the mode of migration and associated arrangement of the actomyosin cytoskeleton. We observed that cells tended to migrate at constant speed, the magnitude of which was dependent on the size of the channels, as was the locomotory strategy adopted by each cell. Two different migration modes were observed, pseudopod-based and bleb-based migration, with bleb based migration being more frequent with increasing confinement and leading to slower migration. Beside the migration mode, we found that the major determinants of cell speed are its protrusion rate, the amount of F-actin at its leading edge and the number of actin foci. Our results highlighted the impact of the microenvironments on cell behavior. Furthermore, we developed a novel quantitative movement analysis platform for mono-dimensional cell migration that allows for standardization and simplification of the experimental conditions and aids investigation of the complex and dynamic processes occurring at the single-cell level.